| http://purl.uniprot.org/citations/10619842 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10619842 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10619842 | http://www.w3.org/2000/01/rdf-schema#comment | "In the yeast Saccharomyces cerevisiae, pre-mRNA 3'-end processing requires six factors: cleavage factor IA (CF IA), cleavage factor IB (CF IB), cleavage factor II (CF II), polyadenylation factor I (PF I), poly(A) polymerase (Pap1p) and poly(A)-binding protein I (Pab1p). We report the characterization of Pfs2p, a WD-repeat protein previously identified in a multiprotein complex carrying PF I-Pap1p activity. The 3'-end-processing defects of pfs2 mutant strains and the results of immunodepletion and immunoinactivation experiments indicate an essential function for Pfs2p in cleavage and polyadenylation. With a one-step affinity purification method that exploits protein A-tagged Pfs2p, we showed that this protein is part of a CF II-PF I complex. Pull-down experiments with GST fusion proteins revealed direct interactions of Pfs2p with subunits of CF II-PF I and CF IA. These results show that Pfs2p plays an essential role in 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex."xsd:string |
| http://purl.uniprot.org/citations/10619842 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/19.1.37"xsd:string |
| http://purl.uniprot.org/citations/10619842 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/19.1.37"xsd:string |
| http://purl.uniprot.org/citations/10619842 | http://purl.uniprot.org/core/author | "Keller W."xsd:string |
| http://purl.uniprot.org/citations/10619842 | http://purl.uniprot.org/core/author | "Keller W."xsd:string |
| http://purl.uniprot.org/citations/10619842 | http://purl.uniprot.org/core/author | "Barabino S.M.L."xsd:string |
| http://purl.uniprot.org/citations/10619842 | http://purl.uniprot.org/core/author | "Barabino S.M.L."xsd:string |
| http://purl.uniprot.org/citations/10619842 | http://purl.uniprot.org/core/author | "Preker P.J."xsd:string |
| http://purl.uniprot.org/citations/10619842 | http://purl.uniprot.org/core/author | "Preker P.J."xsd:string |
| http://purl.uniprot.org/citations/10619842 | http://purl.uniprot.org/core/author | "Ohnacker M."xsd:string |
| http://purl.uniprot.org/citations/10619842 | http://purl.uniprot.org/core/author | "Ohnacker M."xsd:string |
| http://purl.uniprot.org/citations/10619842 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10619842 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10619842 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/10619842 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/10619842 | http://purl.uniprot.org/core/pages | "37-47"xsd:string |
| http://purl.uniprot.org/citations/10619842 | http://purl.uniprot.org/core/pages | "37-47"xsd:string |
| http://purl.uniprot.org/citations/10619842 | http://purl.uniprot.org/core/title | "The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex."xsd:string |
| http://purl.uniprot.org/citations/10619842 | http://purl.uniprot.org/core/title | "The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex."xsd:string |
| http://purl.uniprot.org/citations/10619842 | http://purl.uniprot.org/core/volume | "19"xsd:string |
| http://purl.uniprot.org/citations/10619842 | http://purl.uniprot.org/core/volume | "19"xsd:string |
| http://purl.uniprot.org/citations/10619842 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10619842 |
| http://purl.uniprot.org/citations/10619842 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10619842 |