| http://purl.uniprot.org/citations/10625686 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10625686 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10625686 | http://www.w3.org/2000/01/rdf-schema#comment | "Auxilin is a brain-specific DnaJ homolog that is required for Hsc70 to dissociate clathrin from bovine brain clathrin-coated vesicles. However, Hsc70 is also involved in uncoating clathrin-coated vesicles formed at the plasma membrane of non-neuronal cells suggesting that an auxilin homolog may be required for uncoating in these cells. One candidate is cyclin G-associated kinase (GAK), a 150-kDa protein expressed ubiquitously in various tissues. GAK has a C-terminal domain with high sequence similarity to auxilin; like auxilin this C-terminal domain consists of three subdomains, an N-terminal tensin-like domain, a clathrin-binding domain, and a C-terminal J-domain. Western blot analysis shows that GAK is present in rat liver, bovine testes, and bovine brain clathrin-coated vesicles. More importantly, liver clathrin-coated vesicles, which contain GAK but not auxilin, are uncoated by Hsc70, suggesting that GAK acts as an auxilin homolog in non-neuronal cells. In support of this view, the clathrin-binding domain of GAK alone induces clathrin polymerization into baskets and the combined clathrin-binding domain and J-domain of GAK supports uncoating of AP180-clathrin baskets by Hsc70 at pH 7 and induces Hsc70 binding to clathrin baskets at pH 6. Immunolocalization studies suggest that GAK is a cytosolic protein that is concentrated in the perinuclear region; it appears to be highly associated with the trans-Golgi where the budding of clathrin-coated vesicles occurs. We propose that GAK is a required cofactor for the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells."xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.275.2.1365"xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.275.2.1365"xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/author | "Nojima H."xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/author | "Nojima H."xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/author | "Zhao X."xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/author | "Zhao X."xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/author | "Eisenberg E."xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/author | "Eisenberg E."xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/author | "Greene L.E."xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/author | "Greene L.E."xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/author | "Greener T."xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/author | "Greener T."xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/pages | "1365-1370"xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/pages | "1365-1370"xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/title | "Role of cyclin G-associated kinase in uncoating clathrin-coated vesicles from non-neuronal cells."xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/title | "Role of cyclin G-associated kinase in uncoating clathrin-coated vesicles from non-neuronal cells."xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/volume | "275"xsd:string |
| http://purl.uniprot.org/citations/10625686 | http://purl.uniprot.org/core/volume | "275"xsd:string |