RDF/XMLNTriplesTurtleShow queryShare
SubjectPredicateObject
http://purl.uniprot.org/citations/10627032http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10627032http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10627032http://www.w3.org/2000/01/rdf-schema#comment"After transformation of the Streptomyces lividans chloramphenicol-sensitive, arginine-auxotrophic mutant strain AJ100 with a derivative of plasmid SCP2, some of the regenerated protoplasts contained an 8.2 kb DNA sequence amplified to several hundred copies per chromosome. The corresponding non-amplified sequence, called AUD4, was isolated from a lambda phage genomic library of S. lividans 1326. Two cytosine residues were the only directly repeated nucleotides at the ends of the element, indicating that AUD4 is a class I amplifiable sequence. The element mapped in the AseI-D fragment of the S. lividans chromosome, where other class I amplifications have been described. The complete element was sequenced and 10 ORFs were identified. Some of the deduced proteins are highly conserved in other organisms but a putative function could be attributed to only a few of them. Duplication of AUD4 by integration of an Escherichia coli plasmid carrying various parts of AUD4 and a thiostrepton-resistance gene in S. lividans AJ100, ZX7 or TK64 induced amplification of the integrated plasmid, AUD4 or both at high frequency."xsd:string
http://purl.uniprot.org/citations/10627032http://purl.org/dc/terms/identifier"doi:10.1099/00221287-145-12-3331"xsd:string
http://purl.uniprot.org/citations/10627032http://purl.uniprot.org/core/author"Altenbuchner J."xsd:string
http://purl.uniprot.org/citations/10627032http://purl.uniprot.org/core/author"Altenbuchner J."xsd:string
http://purl.uniprot.org/citations/10627032http://purl.uniprot.org/core/author"Buchler C."xsd:string
http://purl.uniprot.org/citations/10627032http://purl.uniprot.org/core/author"Buchler C."xsd:string
http://purl.uniprot.org/citations/10627032http://purl.uniprot.org/core/author"Schmid E."xsd:string
http://purl.uniprot.org/citations/10627032http://purl.uniprot.org/core/author"Schmid E."xsd:string
http://purl.uniprot.org/citations/10627032http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10627032http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10627032http://purl.uniprot.org/core/name"Microbiology"xsd:string
http://purl.uniprot.org/citations/10627032http://purl.uniprot.org/core/name"Microbiology (Reading)"xsd:string
http://purl.uniprot.org/citations/10627032http://purl.uniprot.org/core/pages"3331-3341"xsd:string
http://purl.uniprot.org/citations/10627032http://purl.uniprot.org/core/pages"3331-3341"xsd:string
http://purl.uniprot.org/citations/10627032http://purl.uniprot.org/core/title"AUD4, a new amplifiable element from Streptomyces lividans."xsd:string
http://purl.uniprot.org/citations/10627032http://purl.uniprot.org/core/title"AUD4, a new amplifiable element from Streptomyces lividans."xsd:string
http://purl.uniprot.org/citations/10627032http://purl.uniprot.org/core/volume"145"xsd:string
http://purl.uniprot.org/citations/10627032http://purl.uniprot.org/core/volume"145"xsd:string
http://purl.uniprot.org/citations/10627032http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/10627032
http://purl.uniprot.org/citations/10627032http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/10627032
http://purl.uniprot.org/citations/10627032http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/10627032
http://purl.uniprot.org/citations/10627032http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/10627032
http://purl.uniprot.org/uniprot/O85696http://purl.uniprot.org/core/citationhttp://purl.uniprot.org/citations/10627032