| http://purl.uniprot.org/citations/10627538 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10627538 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10627538 | http://www.w3.org/2000/01/rdf-schema#comment | "The African swine fever virus (ASFV) genome contains a gene, 9GL, with similarity to yeast ERV1 and ALR genes. ERV1 has been shown to function in oxidative phosphorylation and in cell growth, while ALR has hepatotrophic activity. 9GL encodes a protein of 119 amino acids and was highly conserved at both nucleotide and amino acid levels among all ASFV field isolates examined. Monospecific rabbit polyclonal antibody produced to a glutathione S-transferase-9GL fusion protein specifically immunoprecipitated a 14-kDa protein from macrophage cell cultures infected with the ASFV isolate Malawi Lil-20/1 (MAL). Time course analysis and viral DNA synthesis inhibitor experiments indicated that p14 was a late viral protein. A 9GL gene deletion mutant of MAL (Delta9GL), exhibited a growth defect in macrophages of approximately 2 log(10) units and had a small-plaque phenotype compared to either a revertant (9GL-R) or the parental virus. 9GL affected normal virion maturation; virions containing acentric nucleoid structures comprised 90 to 99% of all virions observed in Delta9GL-infected macrophages. The Delta9GL virus was markedly attenuated in swine. In contrast to 9GL-R infection, where mortality was 100%, all Delta9GL-infected animals survived infection. With the exception of a transient fever response in some animals, Delta9GL-infected animals remained clinically normal and exhibited significant 100-to 10,000-fold reductions in viremia titers. All pigs previously infected with Delta9GL survived infection when subsequently challenged with a lethal dose of virulent parental MAL. Thus, ASFV 9GL gene deletion mutants may prove useful as live-attenuated ASF vaccines."xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.org/dc/terms/identifier | "doi:10.1128/jvi.74.3.1275-1285.2000"xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.org/dc/terms/identifier | "doi:10.1128/jvi.74.3.1275-1285.2000"xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.org/dc/terms/identifier | "doi:10.1128/JVI.74.3.1275-1285.2000"xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.uniprot.org/core/author | "Burrage T.G."xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.uniprot.org/core/author | "Burrage T.G."xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.uniprot.org/core/author | "Kutish G.F."xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.uniprot.org/core/author | "Kutish G.F."xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.uniprot.org/core/author | "Lu Z."xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.uniprot.org/core/author | "Lu Z."xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.uniprot.org/core/author | "Rock D.L."xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.uniprot.org/core/author | "Rock D.L."xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.uniprot.org/core/author | "Neilan J.G."xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.uniprot.org/core/author | "Neilan J.G."xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.uniprot.org/core/author | "Zsak L."xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.uniprot.org/core/author | "Zsak L."xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.uniprot.org/core/author | "Lewis T."xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.uniprot.org/core/author | "Lewis T."xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10627538 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10627538 | http://purl.uniprot.org/core/name | "J. Virol."xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.uniprot.org/core/name | "J. Virol."xsd:string |
| http://purl.uniprot.org/citations/10627538 | http://purl.uniprot.org/core/pages | "1275-1285"xsd:string |