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http://purl.uniprot.org/citations/10643692http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10643692http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10643692http://www.w3.org/2000/01/rdf-schema#comment"

Objectives

To determine human PPARgamma coactivator-1 (PGC-1) amino acid sequence and to study PGC-1 mRNA tissue distribution. PGC-1 is a novel transcriptional coactivator of nuclear receptors that may play a role in the control of thermogenesis.

Subjects

Subcutaneous adipose tissue was obtained from six obese and five lean male subjects. Vastus lateralis skeletal muscle was obtained from seven lean and six obese subjects undergoing a 5-day severe calorie restriction. Other tissue biopsies were from nonobese nondiabetic subjects.

Methods

Human PGC-1 was cloned from a skeletal muscle cDNA library. A reverse transcription-competitive polymerase chain reaction assay was developed to determine PGC-1 mRNA levels in human tissues.

Results

The human amino acid sequence showed 95% identity with mouse PGC-1. PGC-1 mRNA was expressed at very low levels in the small and large intestines and white adipose tissue. Heart, kidney, liver and skeletal muscle showed higher mRNA levels. The degree of obesity did not affect PGC-1 mRNA levels in adipose tissue while lean subjects expressed more PGC-1 mRNA than obese subjects in skeletal muscle. A 5-day severe calorie restriction induced PGC-1 mRNA expression in skeletal muscle of obese but not of lean subjects.

Conclusion

PGC-1 shows a restricted tissue expression that suggests a tissue-specific role in the control of gene transcription and possible interaction with various members of the PPAR family. The lower expression of skeletal muscle PGC-1 in obesity could contribute to an alteration of mitochondrial gene expression."xsd:string
http://purl.uniprot.org/citations/10643692http://purl.org/dc/terms/identifier"doi:10.1038/sj.ijo.0801106"xsd:string
http://purl.uniprot.org/citations/10643692http://purl.org/dc/terms/identifier"doi:10.1038/sj.ijo.0801106"xsd:string
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/author"Andreelli F."xsd:string
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/author"Andreelli F."xsd:string
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/author"Langin D."xsd:string
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/author"Langin D."xsd:string
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/author"Vidal H."xsd:string
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/author"Vidal H."xsd:string
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/author"Laville M."xsd:string
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/author"Laville M."xsd:string
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/author"Larrouy D."xsd:string
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/author"Larrouy D."xsd:string
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/name"Int. J. Obes. Relat. Metab. Disord."xsd:string
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/name"Int. J. Obes. Relat. Metab. Disord."xsd:string
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/pages"1327-1332"xsd:string
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/pages"1327-1332"xsd:string
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/title"Cloning and mRNA tissue distribution of human PPARgamma coactivator-1."xsd:string
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/title"Cloning and mRNA tissue distribution of human PPARgamma coactivator-1."xsd:string
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/volume"23"xsd:string
http://purl.uniprot.org/citations/10643692http://purl.uniprot.org/core/volume"23"xsd:string
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