| http://purl.uniprot.org/citations/10660621 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10660621 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10660621 | http://www.w3.org/2000/01/rdf-schema#comment | "Steroid receptor coactivator-1 (SRC-1) is a member of a coactivator family that enhance the activation of the steroid/nuclear receptor superfamily of ligand-stimulated transcription factors. To study the regulation of SRC-1 by signaling pathways in the cell, the major phosphorylation sites of SRC-1 were identified in COS-1 cells using a combination of in vivo labeling with [(32)P]H(3)PO(4), modified manual Edman degradation, phosphoamino acid analysis, endoproteinase digestion, and mutagenesis of the SRC-1 phosphorylation sites. Seven phosphorylation sites were identified in SRC-1: serine 372, serine 395, serine 517, serine 569, serine 1033, threonine 1179, and serine 1185. All the sites contained consensus sequences for the serine/threonine-proline-directed family of protein kinases, and two sites (serine 395 and threonine 1179) contained a perfect consensus sequence for the mitogen-activated protein kinase family (Erk-1 and Erk-2). Furthermore, Erk-2 phosphorylated threonine 1179 and serine 1185 (and to a lesser extent, serine 395) in vitro, suggesting the importance of this pathway for SRC-1 regulation. Treatment of cells expressing SRC-1 with epidermal growth factor enhanced the ligand-dependent, progesterone receptor-mediated activation of a target reporter gene. These results identify phosphorylation as a regulatory modification of SRC-1 and provide a basis upon which to identify signaling pathways that regulate SRC-1 function and, consequently, modify steroid/nuclear receptor action."xsd:string |
| http://purl.uniprot.org/citations/10660621 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.275.6.4475"xsd:string |
| http://purl.uniprot.org/citations/10660621 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.275.6.4475"xsd:string |
| http://purl.uniprot.org/citations/10660621 | http://purl.uniprot.org/core/author | "Weigel N.L."xsd:string |
| http://purl.uniprot.org/citations/10660621 | http://purl.uniprot.org/core/author | "Weigel N.L."xsd:string |
| http://purl.uniprot.org/citations/10660621 | http://purl.uniprot.org/core/author | "O'Malley B.W."xsd:string |
| http://purl.uniprot.org/citations/10660621 | http://purl.uniprot.org/core/author | "O'Malley B.W."xsd:string |
| http://purl.uniprot.org/citations/10660621 | http://purl.uniprot.org/core/author | "Rowan B.G."xsd:string |
| http://purl.uniprot.org/citations/10660621 | http://purl.uniprot.org/core/author | "Rowan B.G."xsd:string |
| http://purl.uniprot.org/citations/10660621 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10660621 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10660621 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10660621 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10660621 | http://purl.uniprot.org/core/pages | "4475-4483"xsd:string |
| http://purl.uniprot.org/citations/10660621 | http://purl.uniprot.org/core/pages | "4475-4483"xsd:string |
| http://purl.uniprot.org/citations/10660621 | http://purl.uniprot.org/core/title | "Phosphorylation of steroid receptor coactivator-1. Identification of the phosphorylation sites and phosphorylation through the mitogen-activated protein kinase pathway."xsd:string |
| http://purl.uniprot.org/citations/10660621 | http://purl.uniprot.org/core/title | "Phosphorylation of steroid receptor coactivator-1. Identification of the phosphorylation sites and phosphorylation through the mitogen-activated protein kinase pathway."xsd:string |
| http://purl.uniprot.org/citations/10660621 | http://purl.uniprot.org/core/volume | "275"xsd:string |
| http://purl.uniprot.org/citations/10660621 | http://purl.uniprot.org/core/volume | "275"xsd:string |
| http://purl.uniprot.org/citations/10660621 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10660621 |
| http://purl.uniprot.org/citations/10660621 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10660621 |
| http://purl.uniprot.org/citations/10660621 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10660621 |
| http://purl.uniprot.org/citations/10660621 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10660621 |