| http://purl.uniprot.org/citations/10669729 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10669729 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10669729 | http://www.w3.org/2000/01/rdf-schema#comment | "Polyadenylation of mRNA precursors is a two-step reaction requiring multiple protein factors. Cleavage stimulation factor (CstF) is a heterotrimer necessary for the first step, endonucleolytic cleavage, and it plays an important role in determining the efficiency of polyadenylation. Although a considerable amount is known about the RNA binding properties of CstF, the protein-protein interactions required for its assembly and function are poorly understood. We therefore first identified regions of the CstF subunits, CstF-77, CstF-64, and CstF-50, required for interaction with each other. Unexpectedly, small regions of two of the subunits participate in multiple interactions. In CstF-77, a proline-rich domain is necessary not only for binding both other subunits but also for self-association, an interaction consistent with genetic studies in Drosophila. In CstF-64, a small region, highly conserved in metazoa, is responsible for interactions with two proteins, CstF-77 and symplekin, a nuclear protein of previously unknown function. Intriguingly, symplekin has significant similarity to a yeast protein, PTA1, that is a component of the yeast polyadenylation machinery. We show that multiple factors, including CstF, cleavage-polyadenylation specificity factor, and symplekin, can be isolated from cells as part of a large complex. These and other data suggest that symplekin may function in assembly of the polyadenylation machinery."xsd:string |
| http://purl.uniprot.org/citations/10669729 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.20.5.1515-1525.2000"xsd:string |
| http://purl.uniprot.org/citations/10669729 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.20.5.1515-1525.2000"xsd:string |
| http://purl.uniprot.org/citations/10669729 | http://purl.uniprot.org/core/author | "Manley J.L."xsd:string |
| http://purl.uniprot.org/citations/10669729 | http://purl.uniprot.org/core/author | "Manley J.L."xsd:string |
| http://purl.uniprot.org/citations/10669729 | http://purl.uniprot.org/core/author | "Takagaki Y."xsd:string |
| http://purl.uniprot.org/citations/10669729 | http://purl.uniprot.org/core/author | "Takagaki Y."xsd:string |
| http://purl.uniprot.org/citations/10669729 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10669729 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10669729 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/10669729 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/10669729 | http://purl.uniprot.org/core/pages | "1515-1525"xsd:string |
| http://purl.uniprot.org/citations/10669729 | http://purl.uniprot.org/core/pages | "1515-1525"xsd:string |
| http://purl.uniprot.org/citations/10669729 | http://purl.uniprot.org/core/title | "Complex protein interactions within the human polyadenylation machinery identify a novel component."xsd:string |
| http://purl.uniprot.org/citations/10669729 | http://purl.uniprot.org/core/title | "Complex protein interactions within the human polyadenylation machinery identify a novel component."xsd:string |
| http://purl.uniprot.org/citations/10669729 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/10669729 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/10669729 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10669729 |
| http://purl.uniprot.org/citations/10669729 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10669729 |
| http://purl.uniprot.org/citations/10669729 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10669729 |
| http://purl.uniprot.org/citations/10669729 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10669729 |
| http://purl.uniprot.org/uniprot/Q05048 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10669729 |
| http://purl.uniprot.org/uniprot/Q92797 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10669729 |