| http://purl.uniprot.org/citations/10675023 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10675023 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10675023 | http://www.w3.org/2000/01/rdf-schema#comment | "We have constructed a two-dimensional database of the proteome of Haemophilus influenzae, a bacterium of medical interest of which the complete genome, comprising about 1742 open reading frames, has been sequenced. The soluble protein fraction of the microorganism was analyzed by two-dimensional electrophoresis, using immobilized pH gradient strips of various pH regions, gels with different acrylamide concentrations and buffers with different trailing ions. In order to visualize low-copy-number gene products, we employed a series of protein extraction and sample application approaches and several chromatographic steps, including heparin chromatography, chromatofocusing and hydrophobic interaction chromatography. We have also analyzed the cell envelope-bound protein fraction using either immobilized pH gradient strips or a two-detergent system with a cationic detergent in the first and an anionic detergent in the second-dimensional separation. Different proteins (502) were identified by matrix-assisted laser desorption/ionization mass spectrometry and amino acid composition analysis. This is at present one of the largest two-dimensional proteome databases."xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.org/dc/terms/identifier | "doi:10.1002/(sici)1522-2683(20000101)21:2<411::aid-elps411>3.0.co;2-4"xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.org/dc/terms/identifier | "doi:10.1002/(sici)1522-2683(20000101)21:2<411::aid-elps411>3.0.co;2-4"xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/author | "Berndt P."xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/author | "Berndt P."xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/author | "Evers S."xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/author | "Evers S."xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/author | "Fountoulakis M."xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/author | "Fountoulakis M."xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/author | "Gray C."xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/author | "Gray C."xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/author | "Lahm H.W."xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/author | "Lahm H.W."xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/author | "Langen H."xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/author | "Langen H."xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/author | "Takacs B."xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/author | "Takacs B."xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/author | "Wipf B."xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/author | "Wipf B."xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/name | "Electrophoresis"xsd:string |
| http://purl.uniprot.org/citations/10675023 | http://purl.uniprot.org/core/name | "Electrophoresis"xsd:string |