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http://purl.uniprot.org/citations/10678921http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10678921http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10678921http://www.w3.org/2000/01/rdf-schema#comment"Upon iron restriction, Actinobacillus pleuropneumoniae has been shown to express the transferrin-binding proteins TbpB and TbpA, both of which have been implied to be important virulence factors. In order to identify additional iron-regulated proteins, we cloned and analyzed the region upstream of the transferrin-binding protein genes in an A. pleuropneumoniae serotype 7 strain. We located immediately upstream of the tbpB gene two open reading frames which were 43% homologous to the neisserial ExbBD protein genes. By raising specific antibodies, we showed that ExbB is expressed under iron-limiting growth conditions only, and RT-PCR analysis revealed that the exbBD genes and the tbpB gene are transcribed on a single polycistronic mRNA. By constructing an isogenic and nonpolar exbBD mutant, we showed that the exbBD genes are required by A. pleuropneumoniae for utilization of transferrin-bound iron. Using PCR and Western blotting, we showed that the genetic organization found in A. pleuropneumoniae serotype 7 is similar in all 12 A. pleuropneumoniae serotype reference strains."xsd:string
http://purl.uniprot.org/citations/10678921http://purl.org/dc/terms/identifier"doi:10.1128/IAI.68.3.1164-1170.2000"xsd:string
http://purl.uniprot.org/citations/10678921http://purl.org/dc/terms/identifier"doi:10.1128/iai.68.3.1164-1170.2000"xsd:string
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/author"Baltes N."xsd:string
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/author"Baltes N."xsd:string
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/author"Gerlach G.F."xsd:string
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/author"Gerlach G.F."xsd:string
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/author"Oswald W."xsd:string
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/author"Oswald W."xsd:string
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/author"Thiede S."xsd:string
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/author"Thiede S."xsd:string
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/author"Tonpitak W."xsd:string
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/author"Tonpitak W."xsd:string
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/date"2000"xsd:gYear
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/date"2000"xsd:gYear
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/name"Infect. Immun."xsd:string
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/name"Infect Immun"xsd:string
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/pages"1164-1170"xsd:string
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/pages"1164-1170"xsd:string
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/title"Actinobacillus pleuropneumoniae iron transport: a set of exbBD genes is transcriptionally linked to the tbpB gene and required for utilization of transferrin-bound iron."xsd:string
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/title"Actinobacillus pleuropneumoniae iron transport: a set of exbBD genes is transcriptionally linked to the tbpB gene and required for utilization of transferrin-bound iron."xsd:string
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/volume"68"xsd:string
http://purl.uniprot.org/citations/10678921http://purl.uniprot.org/core/volume"68"xsd:string