| http://purl.uniprot.org/citations/10679015 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10679015 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10679015 | http://www.w3.org/2000/01/rdf-schema#comment | "Small nucleolar ribonucleoprotein particles (snoRNPs) mainly catalyze the modification of rRNA. The two major classes of snoRNPs, box H/ACA and box C/D, function in the pseudouridylation and 2'-O-methylation, respectively, of specific nucleotides. The emerging view based on studies in yeast is that each class of snoRNPs is composed of a unique set of proteins. Here we present a characterization of mammalian snoRNPs. We show that the previously characterized NAP57 is specific for box H/ACA snoRNPs, whereas the newly identified NAP65, the rat homologue of yeast Nop5/58p, is a component of the box C/D class. Using coimmunoprecipitation experiments, we show that the nucleolar and coiled-body protein Nopp140 interacts with both classes of snoRNPs. This interaction is corroborated in vivo by the exclusive depletion of snoRNP proteins from nucleoli in cells transfected with a dominant negative Nopp140 construct. Interestingly, RNA polymerase I transcription is arrested in nucleoli depleted of snoRNPs, raising the possibility of a feedback mechanism between rRNA modification and transcription. Moreover, the Nopp140-snoRNP interaction appears to be conserved in yeast, because depletion of Srp40p, the yeast Nopp140 homologue, in a conditional lethal strain induces the loss of box H/ACA small nucleolar RNAs. We propose that Nopp140 functions as a chaperone of snoRNPs in yeast and vertebrate cells."xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.11.2.567"xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.11.2.567"xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/author | "Wang C."xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/author | "Wang C."xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/author | "Yang Y."xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/author | "Yang Y."xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/author | "Dragon F."xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/author | "Dragon F."xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/author | "Meier U.T."xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/author | "Meier U.T."xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/author | "Pogacic V."xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/author | "Pogacic V."xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/author | "Isaac C."xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/author | "Isaac C."xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/name | "Mol. Biol. Cell"xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/name | "Mol. Biol. Cell"xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/pages | "567-577"xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/pages | "567-577"xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/title | "Conserved composition of mammalian box H/ACA and box C/D small nucleolar ribonucleoprotein particles and their interaction with the common factor Nopp140."xsd:string |
| http://purl.uniprot.org/citations/10679015 | http://purl.uniprot.org/core/title | "Conserved composition of mammalian box H/ACA and box C/D small nucleolar ribonucleoprotein particles and their interaction with the common factor Nopp140."xsd:string |