| http://purl.uniprot.org/citations/10681512 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10681512 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10681512 | http://www.w3.org/2000/01/rdf-schema#comment | "17beta-Estradiol (E2) induces expression of several genes via estrogen receptor (ER)-Sp1 protein interactions with GC-rich promoter elements in which Sp1 but not ER binds DNA. This study reports the ligand- and cell context-dependent ER(alpha)/Sp1 and ER(beta)/Sp1 action using an E2-responsive construct (pSp1) containing a GC-rich promoter. Both ER(alpha) and ER(beta) proteins physically interact with Sp1 (coimmunoprecipitation) and preferentially bind to the C-terminal region of this protein in pull-down assays. E2- and antiestrogen-dependent transcriptional activation of ER(alpha)/Sp1 was observed in MCF-7, MDA-MB-231, and LnCaP cells, but not in HeLa cells. E2 did not affect or significantly decrease ER(beta)/Sp1 action, and antiestrogens had minimal effects in the same 4 cell lines. Exchange of activation function-1 (AF-1) domains of ER subtypes gave chimeric ER(alpha/beta) (AF-1alpha/AF-2beta) and ER(beta/alpha) (AF-1beta/AF-2alpha) proteins that resembled wild-type ER (alpha or beta) in terms of physical association with Sp1 protein. Transcriptional activation studies with chimeric ER(beta/alpha) and ER(alpha/beta) showed that only ER(alpha/beta) can activate transcription from an Sp1 element, not ER(beta/alpha). This indicates that the AF-1 domain from ER(alpha) is responsible for activation at an Sp1 element, independent of ER subtype context. In order to further characterize this observation, deletion constructs in the AF-1 domain of both ER(alpha) and ER(alpha/beta) were made, and transactivation studies indicated that the region between amino acids 79 and 117 of this domain is important for activation at an Sp1 element."xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.275.8.5379"xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.275.8.5379"xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/author | "Gustafsson J.A."xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/author | "Gustafsson J.A."xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/author | "Nguyen T."xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/author | "Nguyen T."xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/author | "Wang F."xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/author | "Wang F."xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/author | "Safe S."xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/author | "Safe S."xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/author | "Enmark E."xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/author | "Enmark E."xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/author | "Kuiper G."xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/author | "Kuiper G."xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/author | "Saville B."xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/author | "Saville B."xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/author | "Wormke M."xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/author | "Wormke M."xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10681512 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |