| http://purl.uniprot.org/citations/10688816 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10688816 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10688816 | http://www.w3.org/2000/01/rdf-schema#comment | "Two Notch ligand families, Delta and Serrate/Jagged, have been identified in vertebrates. Members of the Jagged family have been shown to affect in vitro hematopoiesis. To determine whether members of the Delta family might play a similar role in hematopoiesis, we examined the expression of mouse Delta-like-1 (mDll1). mDll1 protein was detected in whole marrow and in a marrow stromal cell line MS-5. At the RNA level, both mDll1 and Notch1 were seen in marrow precursor, differentiated hematopoietic, marrow stromal, and MS-5 cells. We isolated a cDNA encoding the human homologue of mDll1, designated human Delta-like-1 (hDll1). A soluble form of hDll1, hDll1(NDSL), containing the DSL domain and the N-terminal sequences, was expressed and purified from bacteria as a glutathione S-transferase (GST) fusion protein. We observed that hDll1(NDSL) delayed the acquisition of differentiation markers by murine hematopoietic progenitor cells (Lin-) cultured in vitro with cytokines. In addition, it promoted greater expansion (more than 3 times) of the primitive hematopoietic precursor cell population, measured in high-proliferative potential colony assay and day 12 colony-forming unit spleen (CFU-S) assay, than GST controls. We also observed that the percentage of apoptotic cells decreased and that the number of cells in the S-phase of the cell cycle increased in the cultures of Lin(-) cells with hDll1(NDSL). The effects of hDll1(NDSL) were blocked by antibody against the mouse counterpart of hDll1(NDSL), mDll1(NDSL). These observations demonstrate that hDll1 plays a role in mediating cell fate decisions during hematopoiesis. (Blood. 2000;95:1616-1625)"xsd:string |
| http://purl.uniprot.org/citations/10688816 | http://purl.uniprot.org/core/author | "Han W."xsd:string |
| http://purl.uniprot.org/citations/10688816 | http://purl.uniprot.org/core/author | "Han W."xsd:string |
| http://purl.uniprot.org/citations/10688816 | http://purl.uniprot.org/core/author | "Ye Q."xsd:string |
| http://purl.uniprot.org/citations/10688816 | http://purl.uniprot.org/core/author | "Ye Q."xsd:string |
| http://purl.uniprot.org/citations/10688816 | http://purl.uniprot.org/core/author | "Moore M.A.S."xsd:string |
| http://purl.uniprot.org/citations/10688816 | http://purl.uniprot.org/core/author | "Moore M.A.S."xsd:string |
| http://purl.uniprot.org/citations/10688816 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10688816 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10688816 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/10688816 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/10688816 | http://purl.uniprot.org/core/pages | "1616-1625"xsd:string |
| http://purl.uniprot.org/citations/10688816 | http://purl.uniprot.org/core/pages | "1616-1625"xsd:string |
| http://purl.uniprot.org/citations/10688816 | http://purl.uniprot.org/core/title | "A soluble form of human Delta-like-1 inhibits differentiation of hematopoietic progenitor cells."xsd:string |
| http://purl.uniprot.org/citations/10688816 | http://purl.uniprot.org/core/title | "A soluble form of human Delta-like-1 inhibits differentiation of hematopoietic progenitor cells."xsd:string |
| http://purl.uniprot.org/citations/10688816 | http://purl.uniprot.org/core/volume | "95"xsd:string |
| http://purl.uniprot.org/citations/10688816 | http://purl.uniprot.org/core/volume | "95"xsd:string |
| http://purl.uniprot.org/citations/10688816 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10688816 |
| http://purl.uniprot.org/citations/10688816 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10688816 |
| http://purl.uniprot.org/citations/10688816 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10688816 |
| http://purl.uniprot.org/citations/10688816 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10688816 |
| http://purl.uniprot.org/embl-cds/AAF05834.1 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10688816 |
| http://purl.uniprot.org/uniprot/O00548 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10688816 |