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| http://purl.uniprot.org/citations/10710367 | http://www.w3.org/2000/01/rdf-schema#comment | "To study the role of endothelial nitric oxide synthase (eNOS) in cardiac function, we compared eNOS expression, contractility, and relaxation in the left ventricles of wild-type and eNOS-deficient mice. eNOS immunostaining is localized to the macro- and microvascular endothelium throughout the myocardium in wild-type mice and is absent in eNOS-/- mice. Whereas blood pressure is elevated in eNOS-/-mice, baseline cardiac contractility (dP/dt(max)) is similar in wild-type and eNOS-/- mice (9,673 +/- 2, 447 and 9,928 +/-1,566 mmHg/s, respectively). The beta-adrenergic agonist isoproterenol (Iso) at doses of >/=1 ng causes enhanced increases in dP/dt(max) in eNOS-/-mice compared with wild-type controls in vivo (P < 0.01) as well as in Langendorff isolated heart preparations (P < 0.02). beta-Adrenergic receptor binding (B(max)) is not significantly different in the two groups of animals (B(max) = 41.4 +/- 9.4 and 36.1 +/-5.1 fmol/mg for wild-type and eNOS-/-). Iso-stimulated ventricular relaxation is also enhanced in the eNOS-/-mice, as measured by dP/dt(min) in the isolated heart. However, baseline ventricular relaxation is normal in eNOS-/- mice (tau = 5.2 +/- 1.0 and 5.6 +/-1.5 ms for wild-type and eNOS-/-, respectively), whereas it is impaired in wild-type mice after NOS inhibition (tau = 8.3 +/-2.4 ms). cGMP levels in the left ventricle are unaffected by eNOS gene deletion (wild-type: 3.1 +/- 0.8 pmol/mg, eNOS-/-: 3.1 +/-0.6 pmol/mg), leading us to examine the level of another physiological regulator of cGMP. Atrial natriuretic peptide (ANP) expression is markedly upregulated in the eNOS-/-mice, and exogenous ANP restores ventricular relaxation in wild-type mice treated with NOS inhibitors. These results suggest that eNOS attenuates both inotropic and lusitropic responses to beta-adrenergic stimulation, and it also appears to regulate baseline ventricular relaxation in conjunction with ANP."xsd:string |
| http://purl.uniprot.org/citations/10710367 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpheart.2000.278.3.h971"xsd:string |
| http://purl.uniprot.org/citations/10710367 | http://purl.uniprot.org/core/author | "Huang P.L."xsd:string |
| http://purl.uniprot.org/citations/10710367 | http://purl.uniprot.org/core/author | "Fishman M.C."xsd:string |
| http://purl.uniprot.org/citations/10710367 | http://purl.uniprot.org/core/author | "Gyurko R."xsd:string |
| http://purl.uniprot.org/citations/10710367 | http://purl.uniprot.org/core/author | "Kuhlencordt P."xsd:string |
| http://purl.uniprot.org/citations/10710367 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10710367 | http://purl.uniprot.org/core/name | "Am J Physiol Heart Circ Physiol"xsd:string |
| http://purl.uniprot.org/citations/10710367 | http://purl.uniprot.org/core/pages | "H971-81"xsd:string |
| http://purl.uniprot.org/citations/10710367 | http://purl.uniprot.org/core/title | "Modulation of mouse cardiac function in vivo by eNOS and ANP."xsd:string |
| http://purl.uniprot.org/citations/10710367 | http://purl.uniprot.org/core/volume | "278"xsd:string |
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