| http://purl.uniprot.org/citations/10737807 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10737807 | http://www.w3.org/2000/01/rdf-schema#comment | "Synaptotagmin, a synaptic vesicle protein involved in Ca(2+)-regulated exocytosis, displayed direct high affinity interaction with neuronal sodium channels. Monoclonal antibodies directed against synaptotagmins I and II adsorbed in a concentration-dependent and -specific manner [(3)H]saxitoxin prelabeled sodium channels extracted with detergent from nerve endings. Conversely, co-immunoprecipitation of synaptotagmin was achieved by antibodies against sodium channel subunits. Consistent with the co-immunoprecipitation assays, solubilized [(3)H]saxitoxin-prelabeled sodium channels were trapped on immobilized maltose binding protein (MBP)-synaptotagmin I. In vitro recombinant protein assays were employed to identify the interaction site of synaptotagmin I, which was located on the cytoplasmic loop between domains I and II of the sodium channel alphaIIA subunit. The co-immunoprecipitated synaptotagmin-sodium channel complexes were found to be Ca(2+)-dependent; this effect was mimicked by Ba(2+) and Sr(2+) but not Mg(2+). Finally the complex was shown to be distinct from the synaptotagmin-SNARE protein complex that can selectively interact with presynaptic calcium channels (N and P/Q types). Thus, our findings demonstrate an unexpected and direct interaction between sodium channels and synaptotagmin. The Ca(2+)-regulated association between sodium channels and a protein implicated in vesicular fusion may have intriguing consequences for the establishment and regulation of neuronal excitability."xsd:string |
| http://purl.uniprot.org/citations/10737807 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.97.7.3666"xsd:string |
| http://purl.uniprot.org/citations/10737807 | http://purl.uniprot.org/core/author | "Dargent B."xsd:string |
| http://purl.uniprot.org/citations/10737807 | http://purl.uniprot.org/core/author | "Leveque C."xsd:string |
| http://purl.uniprot.org/citations/10737807 | http://purl.uniprot.org/core/author | "Seagar M."xsd:string |
| http://purl.uniprot.org/citations/10737807 | http://purl.uniprot.org/core/author | "Tricaud N."xsd:string |
| http://purl.uniprot.org/citations/10737807 | http://purl.uniprot.org/core/author | "Couraud F."xsd:string |
| http://purl.uniprot.org/citations/10737807 | http://purl.uniprot.org/core/author | "Sampo B."xsd:string |
| http://purl.uniprot.org/citations/10737807 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10737807 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/10737807 | http://purl.uniprot.org/core/pages | "3666-3671"xsd:string |
| http://purl.uniprot.org/citations/10737807 | http://purl.uniprot.org/core/title | "Direct interaction between synaptotagmin and the intracellular loop I-II of neuronal voltage-sensitive sodium channels."xsd:string |
| http://purl.uniprot.org/citations/10737807 | http://purl.uniprot.org/core/volume | "97"xsd:string |
| http://purl.uniprot.org/citations/10737807 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10737807 |
| http://purl.uniprot.org/citations/10737807 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10737807 |
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| http://purl.uniprot.org/uniprot/#_A0A0G2K207-mappedCitation-10737807 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10737807 |
| http://purl.uniprot.org/uniprot/#_A0A8I6GFG6-mappedCitation-10737807 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10737807 |
| http://purl.uniprot.org/uniprot/#_A0A8I6GL05-mappedCitation-10737807 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10737807 |
| http://purl.uniprot.org/uniprot/#_A0A8L2R1E5-mappedCitation-10737807 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10737807 |
| http://purl.uniprot.org/uniprot/#_A0A8I5YBA6-mappedCitation-10737807 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10737807 |
| http://purl.uniprot.org/uniprot/#_A9JQD7-mappedCitation-10737807 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10737807 |
| http://purl.uniprot.org/uniprot/#_P21707-mappedCitation-10737807 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10737807 |
| http://purl.uniprot.org/uniprot/#_F1M9G9-mappedCitation-10737807 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10737807 |