| http://purl.uniprot.org/citations/10744151 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10744151 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10744151 | http://www.w3.org/2000/01/rdf-schema#comment | "Three chromatographically distinct forms of a novel fibrinogen-clotting serine endopeptidase, TL-BJ1, 2 and 3, were purified from the venom of Bothrops jararaca by a combination of ammonium sulfate precipitation and chromatographic steps. The three forms of TL-BJ have similar amidolytic and plasma coagulating activities. TL-BJ 1, TL-BJ 2 and TL-BJ 3 cause the specific clotting of fibrinogen with release of fibrinopeptide A, the specific activities are 16.8 NIH U/mg (TL-BJ 1), 16.7 NIH U/mg (TL-BJ 2) and 20.8 NIH U/mg (TL-BJ 3). The most sensitive chromogenic substrates for measuring the amidolytic activity of TL-BJ 3 were D-Pro-Phe-Arg-pNA, D-Phe-pipecolyl-Arg-pNA and Z-D-Arg-Gly-Arg-pNA. The amidolytic and coagulant activities of TL-BJ were inhibited by phenylmethylsulfonyl fluoride but not by hirudin. Benzamidine derivatives, which are competitive inhibitors of trypsin-like serine endopeptidases, also inhibited the amidolytic activity of TL-BJ. In SDS/PAGE the main bands of TL-BJ 1, 2 and 3 showed molecular masses of 30 kDa, 31 kDa and 32 kDa. Upon incubation with N-glycosidase F only TL-BJ 3 remained unchanged, whereas TL-BJ 1 and TL-BJ 2 showed products with molecular masses around 23 kDa. Thus, TL-BJ 3 does not seem to be N-glycosylated. The N-terminal amino acid sequences of TL-BJ 2 and TL-BJ 3 are identical while TL-BJ 1 has five substitutions."xsd:string |
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| http://purl.uniprot.org/citations/10744151 | http://purl.uniprot.org/core/author | "Mentele R."xsd:string |
| http://purl.uniprot.org/citations/10744151 | http://purl.uniprot.org/core/author | "Camargo A.C.M."xsd:string |
| http://purl.uniprot.org/citations/10744151 | http://purl.uniprot.org/core/author | "Camargo A.C.M."xsd:string |
| http://purl.uniprot.org/citations/10744151 | http://purl.uniprot.org/core/author | "Sampaio C.A.M."xsd:string |
| http://purl.uniprot.org/citations/10744151 | http://purl.uniprot.org/core/author | "Sampaio C.A.M."xsd:string |
| http://purl.uniprot.org/citations/10744151 | http://purl.uniprot.org/core/author | "Fink E."xsd:string |
| http://purl.uniprot.org/citations/10744151 | http://purl.uniprot.org/core/author | "Fink E."xsd:string |
| http://purl.uniprot.org/citations/10744151 | http://purl.uniprot.org/core/author | "Serrano S.M.T."xsd:string |
| http://purl.uniprot.org/citations/10744151 | http://purl.uniprot.org/core/author | "Serrano S.M.T."xsd:string |
| http://purl.uniprot.org/citations/10744151 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10744151 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10744151 | http://purl.uniprot.org/core/name | "Thromb. Haemost."xsd:string |
| http://purl.uniprot.org/citations/10744151 | http://purl.uniprot.org/core/name | "Thromb. Haemost."xsd:string |
| http://purl.uniprot.org/citations/10744151 | http://purl.uniprot.org/core/pages | "438-444"xsd:string |
| http://purl.uniprot.org/citations/10744151 | http://purl.uniprot.org/core/pages | "438-444"xsd:string |
| http://purl.uniprot.org/citations/10744151 | http://purl.uniprot.org/core/title | "A novel fibrinogen-clotting enzyme, TL-BJ, from the venom of the snake Bothrops jararaca: purification and characterization."xsd:string |
| http://purl.uniprot.org/citations/10744151 | http://purl.uniprot.org/core/title | "A novel fibrinogen-clotting enzyme, TL-BJ, from the venom of the snake Bothrops jararaca: purification and characterization."xsd:string |
| http://purl.uniprot.org/citations/10744151 | http://purl.uniprot.org/core/volume | "83"xsd:string |
| http://purl.uniprot.org/citations/10744151 | http://purl.uniprot.org/core/volume | "83"xsd:string |
| http://purl.uniprot.org/citations/10744151 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10744151 |
| http://purl.uniprot.org/citations/10744151 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10744151 |