| http://purl.uniprot.org/citations/10758506 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10758506 | http://www.w3.org/2000/01/rdf-schema#comment | "The growth substance auxin mediates many cellular processes, including division, elongation and differentiation. PSIAA6 is a member of the Aux/IAA family of short-lived putative transcriptional regulators that share four conserved domains and whose mRNAs are rapidly induced in the presence of auxin. Here PSIAA6 was shown to serve as a dominant transferable degradation signal when present as a translational fusion with firefly luciferase (LUC), with an in vivo half-life of 13.5 min in transgenic Arabidopsis seedlings. In a transient assay system in tobacco protoplasts using steady-state differences as an indirect measure of protein half-life, LUC fusions with full-length PSIAA6 and IAA1, an Aux/IAA protein from Arabidopsis, resulted in protein accumulations that were 3.5 and 1. 0%, respectively, of that with LUC alone. An N-terminal region spanning conserved domain II of PSIAA6 containing amino acids 18-73 was shown to contain the necessary cis-acting element to confer low protein accumulation onto LUC, while a fusion protein with PSIAA6 amino acids 71-179 had only a slight effect. Single amino acid substitutions of PSIAA6 in conserved domain II, equivalent to those found in two alleles of axr3, a gene that encodes Aux/IAA protein IAA17, resulted in a greater than 50-fold increase in protein accumulation. Thus, the same mutations resulting in an altered auxin response phenotype increase Aux/IAA protein accumulation, providing a direct link between these two processes. In support of this model, transgenic plants engineered to over-express IAA17 have an axr3-like phenotype. Together, these data suggest that rapid degradation of Aux/IAA proteins is necessary for a normal auxin response."xsd:string |
| http://purl.uniprot.org/citations/10758506 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1365-313x.2000.00703.x"xsd:string |
| http://purl.uniprot.org/citations/10758506 | http://purl.uniprot.org/core/author | "Theologis A."xsd:string |
| http://purl.uniprot.org/citations/10758506 | http://purl.uniprot.org/core/author | "Callis J."xsd:string |
| http://purl.uniprot.org/citations/10758506 | http://purl.uniprot.org/core/author | "Ramos J."xsd:string |
| http://purl.uniprot.org/citations/10758506 | http://purl.uniprot.org/core/author | "Leyser O."xsd:string |
| http://purl.uniprot.org/citations/10758506 | http://purl.uniprot.org/core/author | "Rouse D."xsd:string |
| http://purl.uniprot.org/citations/10758506 | http://purl.uniprot.org/core/author | "Worley C.K."xsd:string |
| http://purl.uniprot.org/citations/10758506 | http://purl.uniprot.org/core/author | "Zenser N."xsd:string |
| http://purl.uniprot.org/citations/10758506 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10758506 | http://purl.uniprot.org/core/name | "Plant J"xsd:string |
| http://purl.uniprot.org/citations/10758506 | http://purl.uniprot.org/core/pages | "553-562"xsd:string |
| http://purl.uniprot.org/citations/10758506 | http://purl.uniprot.org/core/title | "Degradation of Aux/IAA proteins is essential for normal auxin signalling."xsd:string |
| http://purl.uniprot.org/citations/10758506 | http://purl.uniprot.org/core/volume | "21"xsd:string |
| http://purl.uniprot.org/citations/10758506 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10758506 |
| http://purl.uniprot.org/citations/10758506 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10758506 |
| http://purl.uniprot.org/uniprot/#_Q67YC2-mappedCitation-10758506 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10758506 |
| http://purl.uniprot.org/uniprot/#_P49677-mappedCitation-10758506 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10758506 |
| http://purl.uniprot.org/uniprot/#_P93830-mappedCitation-10758506 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10758506 |
| http://purl.uniprot.org/uniprot/P93830 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/10758506 |
| http://purl.uniprot.org/uniprot/P49677 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/10758506 |
| http://purl.uniprot.org/uniprot/Q67YC2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/10758506 |