| http://purl.uniprot.org/citations/10760132 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10760132 | http://www.w3.org/2000/01/rdf-schema#comment | "The Rab/Ypt small G proteins are essential for intracellular vesicle trafficking in mammals and yeast. The vesicle-docking process requires that Ypt proteins are located in the vesicle membrane. C-terminal geranylgeranyl anchors mediate the membrane attachment of these proteins. The Rab escort protein (REP) is essential for the recognition of Rab/Ypt small G proteins by geranylgeranyltransferase II (GGTase II) and for their delivery to acceptor membranes. What effect an alteration in the levels of prenylated Rab/Ypt proteins has on vesicle transport or other cellular processes is so far unknown. Here, we report the characterization of a yeast REP mutant, mrs6-2, in which reduced prenylation of Ypt proteins occurs even at the permissive temperature. A shift to the restrictive temperature does not alter exponential growth during the first 3 h. The amount of Sec4p, but not Ypt1p, bound to vesicle membranes is reduced 2.5 h after the shift compared with wild-type or mrs6-2 cells incubated at 25 degrees C. In addition, vesicles fail to be polarized towards the bud and small budded binucleate cells accumulate at this time point. Growth in 1 M sorbitol or overexpression of MLC1, encoding a myosin light chain able to bind the unconventional type V myosin Myo2, or of genes involved in cell wall maintenance, such as SLG1, GFA1 and LRE1, suppresses mrs6-2 thermosensitivity. Our data suggest that, at least at high temperature, a critical minimal level of Ypt protein prenylation is required for maintaining vesicle polarization."xsd:string |
| http://purl.uniprot.org/citations/10760132 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1365-2958.2000.01782.x"xsd:string |
| http://purl.uniprot.org/citations/10760132 | http://purl.uniprot.org/core/author | "Kohlwein S.D."xsd:string |
| http://purl.uniprot.org/citations/10760132 | http://purl.uniprot.org/core/author | "Schweyen R.J."xsd:string |
| http://purl.uniprot.org/citations/10760132 | http://purl.uniprot.org/core/author | "Wagner W."xsd:string |
| http://purl.uniprot.org/citations/10760132 | http://purl.uniprot.org/core/author | "Ragnini A."xsd:string |
| http://purl.uniprot.org/citations/10760132 | http://purl.uniprot.org/core/author | "Bauer B.E."xsd:string |
| http://purl.uniprot.org/citations/10760132 | http://purl.uniprot.org/core/author | "Bialek-Wyrzykowska U."xsd:string |
| http://purl.uniprot.org/citations/10760132 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10760132 | http://purl.uniprot.org/core/name | "Mol Microbiol"xsd:string |
| http://purl.uniprot.org/citations/10760132 | http://purl.uniprot.org/core/pages | "1295-1311"xsd:string |
| http://purl.uniprot.org/citations/10760132 | http://purl.uniprot.org/core/title | "Low levels of Ypt protein prenylation cause vesicle polarization defects and thermosensitive growth that can be suppressed by genes involved in cell wall maintenance."xsd:string |
| http://purl.uniprot.org/citations/10760132 | http://purl.uniprot.org/core/volume | "35"xsd:string |
| http://purl.uniprot.org/citations/10760132 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10760132 |
| http://purl.uniprot.org/citations/10760132 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10760132 |
| http://purl.uniprot.org/uniprot/#_P01123-mappedCitation-10760132 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10760132 |
| http://purl.uniprot.org/uniprot/#_P39958-mappedCitation-10760132 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10760132 |
| http://purl.uniprot.org/uniprot/#_P17065-mappedCitation-10760132 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10760132 |
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| http://purl.uniprot.org/uniprot/#_Q00772-mappedCitation-10760132 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10760132 |
| http://purl.uniprot.org/uniprot/#_P25579-mappedCitation-10760132 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10760132 |
| http://purl.uniprot.org/uniprot/#_P32844-mappedCitation-10760132 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10760132 |
| http://purl.uniprot.org/uniprot/#_P32864-mappedCitation-10760132 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10760132 |
| http://purl.uniprot.org/uniprot/#_P53141-mappedCitation-10760132 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10760132 |