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http://purl.uniprot.org/citations/10769135http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10769135http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10769135http://www.w3.org/2000/01/rdf-schema#comment"We report a multifaceted study of the active site region of human pancreatic alpha-amylase. Through a series of novel kinetic analyses using malto-oligosaccharides and malto-oligosaccharyl fluorides, an overall cleavage action pattern for this enzyme has been developed. The preferred binding/cleavage mode occurs when a maltose residue serves as the leaving group (aglycone sites +1 and +2) and there are three sugars in the glycon (-1, -2, -3) sites. Overall it appears that five binding subsites span the active site, although an additional glycon subsite appears to be a significant factor in the binding of longer substrates. Kinetic parameters for the cleavage of substrates modified at the 2 and 4' ' positions also highlight the importance of these hydroxyl groups for catalysis and identify the rate-determining step. Further kinetic and structural studies pinpoint Asp197 as being the likely nucleophile in catalysis, with substitution of this residue leading to an approximately 10(6)-fold drop in catalytic activity. Structural studies show that the original pseudo-tetrasaccharide structure of acarbose is modified upon binding, presumably through a series of hydrolysis and transglycosylation reactions. The end result is a pseudo-pentasaccharide moiety that spans the active site region with its N-linked "glycosidic" bond positioned at the normal site of cleavage. Interestingly, the side chains of Glu233 and Asp300, along with a water molecule, are aligned about the inhibitor N-linked glycosidic bond in a manner suggesting that these might act individually or collectively in the role of acid/base catalyst in the reaction mechanism. Indeed, kinetic analyses show that substitution of the side chains of either Glu233 or Asp300 leads to as much as a approximately 10(3)-fold decrease in catalytic activity. Structural analyses of the Asp300Asn variant of human pancreatic alpha-amylase and its complex with acarbose clearly demonstrate the importance of Asp300 to the mode of inhibitor binding."xsd:string
http://purl.uniprot.org/citations/10769135http://purl.org/dc/terms/identifier"doi:10.1021/bi9921182"xsd:string
http://purl.uniprot.org/citations/10769135http://purl.org/dc/terms/identifier"doi:10.1021/bi9921182"xsd:string
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/author"Overall C.M."xsd:string
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/author"Overall C.M."xsd:string
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/author"Wang Y."xsd:string
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/author"Wang Y."xsd:string
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/author"Withers S.G."xsd:string
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/author"Withers S.G."xsd:string
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/author"Nguyen N.T."xsd:string
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/author"Nguyen N.T."xsd:string
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/author"Braun C."xsd:string
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/author"Braun C."xsd:string
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/author"Brayer G.D."xsd:string
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/author"Brayer G.D."xsd:string
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/author"Maurus R."xsd:string
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/author"Maurus R."xsd:string
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/author"Rydberg E.H."xsd:string
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/author"Rydberg E.H."xsd:string
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/author"Sidhu G."xsd:string
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/author"Sidhu G."xsd:string
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/date"2000"xsd:gYear
http://purl.uniprot.org/citations/10769135http://purl.uniprot.org/core/date"2000"xsd:gYear
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