| http://purl.uniprot.org/citations/10775271 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10775271 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10775271 | http://www.w3.org/2000/01/rdf-schema#comment | "The MSL5 gene, which codes for the splicing factor BBP/ScSF1, is essential in Saccharomyces cerevisiae, yet previous analyses failed to reveal a defect in assembly of (pre)-spliceosomes or in vitro splicing associated with its depletion. We generated 11 temperature-sensitive (ts) mutants and one cold-sensitive (cs) mutant in the corresponding gene and analyzed their phenotypes. While all mutants were blocked in the formation of commitment complex 2 (CC2) at non-permissive and permissive temperature, the ts mutants showed no defect in spliceosome formation and splicing in vitro. The cs mutant was defective in (pre)-spliceosome formation, but residual splicing activity could be detected. In vivo splicing of reporters carrying introns weakened by mutations in the 5' splice site and/or in the branchpoint region was affected in all mutants. Pre-mRNA leakage to the cytoplasm was strongly increased (up to 40-fold) in the mutants. A combination of ts mutants with a disruption of upf1, a gene involved in nonsense-mediated decay, resulted in a specific synthetic growth phenotype, suggesting that the essential function of SF1 in yeast could be related to the retention of pre-mRNA in the nucleus."xsd:string |
| http://purl.uniprot.org/citations/10775271 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/19.8.1873"xsd:string |
| http://purl.uniprot.org/citations/10775271 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/19.8.1873"xsd:string |
| http://purl.uniprot.org/citations/10775271 | http://purl.uniprot.org/core/author | "Seraphin B."xsd:string |
| http://purl.uniprot.org/citations/10775271 | http://purl.uniprot.org/core/author | "Seraphin B."xsd:string |
| http://purl.uniprot.org/citations/10775271 | http://purl.uniprot.org/core/author | "Rutz B."xsd:string |
| http://purl.uniprot.org/citations/10775271 | http://purl.uniprot.org/core/author | "Rutz B."xsd:string |
| http://purl.uniprot.org/citations/10775271 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10775271 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10775271 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/10775271 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/10775271 | http://purl.uniprot.org/core/pages | "1873-1886"xsd:string |
| http://purl.uniprot.org/citations/10775271 | http://purl.uniprot.org/core/pages | "1873-1886"xsd:string |
| http://purl.uniprot.org/citations/10775271 | http://purl.uniprot.org/core/title | "A dual role for BBP/ScSF1 in nuclear pre-mRNA retention and splicing."xsd:string |
| http://purl.uniprot.org/citations/10775271 | http://purl.uniprot.org/core/title | "A dual role for BBP/ScSF1 in nuclear pre-mRNA retention and splicing."xsd:string |
| http://purl.uniprot.org/citations/10775271 | http://purl.uniprot.org/core/volume | "19"xsd:string |
| http://purl.uniprot.org/citations/10775271 | http://purl.uniprot.org/core/volume | "19"xsd:string |
| http://purl.uniprot.org/citations/10775271 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10775271 |
| http://purl.uniprot.org/citations/10775271 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10775271 |
| http://purl.uniprot.org/citations/10775271 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10775271 |
| http://purl.uniprot.org/citations/10775271 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10775271 |
| http://purl.uniprot.org/uniprot/Q12186 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10775271 |
| http://purl.uniprot.org/uniprot/Q12186#attribution-A238B6B024F21B76FFC61BC966B794EA | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/10775271 |