| http://purl.uniprot.org/citations/10781591 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10781591 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10781591 | http://www.w3.org/2000/01/rdf-schema#comment | "Editing of apolipoprotein B (apoB) mRNA requires the catalytic component APOBEC-1 together with "auxiliary" proteins that have not been conclusively characterized so far. Here we report the purification of these additional components of the apoB mRNA editing enzyme-complex from rat liver and the cDNA cloning of the novel APOBEC-1-stimulating protein (ASP). Two proteins copurified into the final active fraction and were characterized by peptide sequencing and mass spectrometry: KSRP, a 75-kDa protein originally described as a splicing regulating factor, and ASP, a hitherto unknown 65-kDa protein. Separation of these two proteins resulted in a reduction of APOBEC-1-stimulating activity. ASP represents a novel type of RNA-binding protein and contains three single-stranded RNA-binding domains in the amino-terminal half and a putative double-stranded RNA-binding domain at the carboxyl terminus. Purified recombinant glutathione S-transferase (GST)-ASP, but not recombinant GST-KSRP, stimulated recombinant GST-APOBEC-1 to edit apoB RNA in vitro. These data demonstrate that ASP is the second essential component of the apoB mRNA editing enzyme-complex. In rat liver, ASP is apparently associated with KSRP, which may confer stability to the editing enzyme-complex with its substrate apoB RNA serving as an additional auxiliary component."xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m001786200"xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m001786200"xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/author | "Apostel F."xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/author | "Apostel F."xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/author | "Buck F."xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/author | "Buck F."xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/author | "Diehl I."xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/author | "Diehl I."xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/author | "Greeve J."xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/author | "Greeve J."xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/author | "Kirsten R."xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/author | "Kirsten R."xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/author | "Lellek H."xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/author | "Lellek H."xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/pages | "19848-19856"xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/pages | "19848-19856"xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/title | "Purification and molecular cloning of a novel essential component of the apolipoprotein B mRNA editing enzyme-complex."xsd:string |
| http://purl.uniprot.org/citations/10781591 | http://purl.uniprot.org/core/title | "Purification and molecular cloning of a novel essential component of the apolipoprotein B mRNA editing enzyme-complex."xsd:string |