| http://purl.uniprot.org/citations/10805723 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10805723 | http://www.w3.org/2000/01/rdf-schema#comment | "In all eukaryotes, the initiation of DNA synthesis requires the formation of prereplicative complexes (pre-RCs) on replication origins, followed by their activation by two S-T protein kinases, an S-phase cyclin-dependent kinase (S-CDK) and a homologue of yeast Dbf4-Cdc7 kinase (Dbf4p-dependent kinase [DDK]). Here, we show that yeast DDK activity is cell cycle regulated, though less tightly than that of the S-CDK Clb5-Cdk1, and peaks during S phase in correlation with Dbf4p levels. Dbf4p is short-lived throughout the cell cycle, but its instability is accentuated during G(1) by the anaphase-promoting complex. Downregulating DDK activity is physiologically important, as joint Cdc7p and Dbf4p overexpression is lethal. Because pre-RC formation is a highly ordered process, we asked whether S-CDK and DDK need also to function in a specific order for the firing of origins. We found that both kinases are activated independently, but we show that DDK can perform its function for DNA replication only after S-CDKs have been activated. Cdc45p, a protein needed for initiation, binds tightly to chromatin only after S-CDK activation (L. Zou and B. Stillman, Science 280:593-596, 1998). We show that Cdc45p is phosphorylated by DDK in vitro, suggesting that it might be one of DDK's critical substrates after S-CDK activation. Linking the origin-bound DDK to the tightly regulated S-CDK in a dependent sequence of events may ensure that DNA replication initiates only at the right time and place."xsd:string |
| http://purl.uniprot.org/citations/10805723 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.20.11.3795-3806.2000"xsd:string |
| http://purl.uniprot.org/citations/10805723 | http://purl.uniprot.org/core/author | "Schwob E."xsd:string |
| http://purl.uniprot.org/citations/10805723 | http://purl.uniprot.org/core/author | "Zarzov P."xsd:string |
| http://purl.uniprot.org/citations/10805723 | http://purl.uniprot.org/core/author | "Della Seta F."xsd:string |
| http://purl.uniprot.org/citations/10805723 | http://purl.uniprot.org/core/author | "Nougarede R."xsd:string |
| http://purl.uniprot.org/citations/10805723 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10805723 | http://purl.uniprot.org/core/name | "Mol Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/10805723 | http://purl.uniprot.org/core/pages | "3795-3806"xsd:string |
| http://purl.uniprot.org/citations/10805723 | http://purl.uniprot.org/core/title | "Hierarchy of S-phase-promoting factors: yeast Dbf4-Cdc7 kinase requires prior S-phase cyclin-dependent kinase activation."xsd:string |
| http://purl.uniprot.org/citations/10805723 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/10805723 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10805723 |
| http://purl.uniprot.org/citations/10805723 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10805723 |
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| http://purl.uniprot.org/uniprot/#_Q08032-mappedCitation-10805723 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10805723 |
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| http://purl.uniprot.org/uniprot/#_P06243-mappedCitation-10805723 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10805723 |
| http://purl.uniprot.org/uniprot/#_P30283-mappedCitation-10805723 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10805723 |
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| http://purl.uniprot.org/uniprot/#_P32943-mappedCitation-10805723 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10805723 |
| http://purl.uniprot.org/uniprot/P32325 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/10805723 |
| http://purl.uniprot.org/uniprot/P32943 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/10805723 |
| http://purl.uniprot.org/uniprot/P30283 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/10805723 |
| http://purl.uniprot.org/uniprot/P06243 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/10805723 |