| http://purl.uniprot.org/citations/10806190 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10806190 | http://www.w3.org/2000/01/rdf-schema#comment | "Werner's syndrome is a potential model of accelerated human aging. The gene responsible for Werner's syndrome encodes a protein that has a helicase domain homologous to Escherichia coli RecQ. To identify binding partners that regulate the function in concert with Wrn, we screened for proteins using the yeast two-hybrid system with mouse Wrn as bait and found three. One was a novel protein, and the other two were mouse Ubc9 and SUMO-1. Ubc9 also interacted with the mouse homologue of the Bloom's syndrome gene product, another eukaryotic RecQ-type helicase, but not mouse DNA helicase Q1/RecQL (RecQL1). Deletion experiments indicated that both proteins interacted with the N-terminal segment of Wrn (amino acid 272-514). The interaction between Wrn and SUMO-1 was weaker than that between Wrn and Ubc9. Positive interaction was observed in the heterogeneous combination of Wrn and yeast Ubc9 (yUbc9), as well as yUbc9 and SUMO-1, in the two-hybrid system. The interaction between yUbc9 and SUMO-1 was abolished by deleting the C-terminal Gly residue of SUMO-1, which is reportedly required for the formation of Ubc9-SUMO-1 thioester linkage. The interaction of Wrn and SUMO-1 was also abolished by deleting the Gly residue, indicating that the interaction of Wrn and SUMO-1 is mediated by yUbc9 in the two-hybrid system. Finally, we confirmed by immunoblotting with an anti-SUMO-1 antibody that Wrn was covalently attached with SUMO-1."xsd:string |
| http://purl.uniprot.org/citations/10806190 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.c000273200"xsd:string |
| http://purl.uniprot.org/citations/10806190 | http://purl.uniprot.org/core/author | "Seki M."xsd:string |
| http://purl.uniprot.org/citations/10806190 | http://purl.uniprot.org/core/author | "Seki T."xsd:string |
| http://purl.uniprot.org/citations/10806190 | http://purl.uniprot.org/core/author | "Furuichi Y."xsd:string |
| http://purl.uniprot.org/citations/10806190 | http://purl.uniprot.org/core/author | "Enomoto T."xsd:string |
| http://purl.uniprot.org/citations/10806190 | http://purl.uniprot.org/core/author | "Saitoh H."xsd:string |
| http://purl.uniprot.org/citations/10806190 | http://purl.uniprot.org/core/author | "Wang W.S."xsd:string |
| http://purl.uniprot.org/citations/10806190 | http://purl.uniprot.org/core/author | "Imamura O."xsd:string |
| http://purl.uniprot.org/citations/10806190 | http://purl.uniprot.org/core/author | "Kawabe Y."xsd:string |
| http://purl.uniprot.org/citations/10806190 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10806190 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/10806190 | http://purl.uniprot.org/core/pages | "20963-20966"xsd:string |
| http://purl.uniprot.org/citations/10806190 | http://purl.uniprot.org/core/title | "Covalent modification of the Werner's syndrome gene product with the ubiquitin-related protein, SUMO-1."xsd:string |
| http://purl.uniprot.org/citations/10806190 | http://purl.uniprot.org/core/volume | "275"xsd:string |
| http://purl.uniprot.org/citations/10806190 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10806190 |
| http://purl.uniprot.org/citations/10806190 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10806190 |
| http://purl.uniprot.org/uniprot/P63280#attribution-342BE00148E5B04A98F988B9D137DF0C | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/10806190 |
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| http://purl.uniprot.org/uniprot/#_G3UWJ1-mappedCitation-10806190 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10806190 |
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| http://purl.uniprot.org/uniprot/#_G3UYP0-mappedCitation-10806190 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10806190 |