| http://purl.uniprot.org/citations/10818110 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10818110 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10818110 | http://www.w3.org/2000/01/rdf-schema#comment | "The ras-related GTPase rab5 is rate-limiting for homotypic early endosome fusion. We used a yeast two-hybrid screen to identify a rab5 interacting protein, rab5ip. The cDNA sequence encodes a ubiquitous 75-kDa protein with an N-terminal transmembrane domain (TM), a central coiled-coil structure, and a C-terminal region homologous to several centrosome-associated proteins. rab5ip lacking the transmembrane domain (rab5ipTM(-)) had a greater affinity in vitro for rab5-guanosine 5'-O-2-(thio)diphosphate than for rab5-guanosine 5'-3-O-(thio)triphosphate. In transfected HeLa cells, rab5ipTM(-) was partly cytosolic and localized (by immunofluorescence) with a rab5 mutant believed to be in a GDP conformation (GFP-rab5(G78A)) but not with GFP-rab5(Q79L), a GTPase-deficient mutant. rab5ip with the transmembrane domain (rab5ipTM(+)) was completely associated with the particulate fraction and localized extensively with GFP-rab5(wt) in punctate endosome-like structures. Overexpression of rab5ipTM(+) using Sindbis virus stimulated the accumulation of fluid-phase horseradish peroxidase by BHK-21 cells, and homotypic endosome fusion in vitro was inhibited by antibody against rab5ip. rab5ipTM(-) inhibited rab5(wt)-stimulated endosome fusion but did not inhibit fusion stimulated by rab5(Q79L). rab5ip represents a novel rab5 interacting protein that may function on endocytic vesicles as a receptor for rab5-GDP and participate in the activation of rab5."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m909600199"xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m909600199"xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Liu X."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Liu X."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Dai W."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Dai W."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Stahl P.D."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Stahl P.D."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Nikolova L."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Nikolova L."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Aballay A."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Aballay A."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Barbieri M.A."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Barbieri M.A."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Dickey B.F."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Dickey B.F."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Knoll B.J."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Knoll B.J."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Baughn R.E."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Baughn R.E."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Hall H.S."xsd:string |
| http://purl.uniprot.org/citations/10818110 | http://purl.uniprot.org/core/author | "Hall H.S."xsd:string |