| http://purl.uniprot.org/citations/10831593 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10831593 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10831593 | http://www.w3.org/2000/01/rdf-schema#comment | "Membrane-type serine protease 1 (MT-SP1) was recently cloned, and we now report its biochemical characterization. MT-SP1 is predicted to be a type II transmembrane protein with an extracellular protease domain. This localization was experimentally verified using immunofluorescent microscopy and a cell-surface biotinylation technique. The substrate specificity of MT-SP1 was determined using a positional scanning-synthetic combinatorial library and substrate phage techniques. The preferred cleavage sequences were found to be (P4-(Arg/Lys)P3-(X)P2-(Ser)P1-(Arg)P1'-(Ala)) and (P4-(X)P3-(Arg/Lys)P2-(Ser)P1(Arg) P1'(Ala)), where X is a non-basic amino acid. Protease-activated receptor 2 (PAR2) and single-chain urokinase-type plasminogen activator are proteins that are localized to the extracellular surface and contain the preferred MT-SP1 cleavage sequence. The ability of MT-SP1 to activate PARs was assessed by exposing PAR-expressing Xenopus oocytes to the soluble MT-SP1 protease domain. The latter triggered calcium signaling in PAR2-expressing oocytes at 10 nm but failed to trigger calcium signaling in oocytes expressing PAR1, PAR3, or PAR4 at 100 nm. Single-chain urokinase-type plasminogen activator was activated using catalytic amounts of MT-SP1 (1 nm), but plasminogen was not cleaved under similar conditions. The membrane localization of MT-SP1 and its affinity for these key extracellular substrates suggests a role of the proteolytic activity in regulatory events."xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m002941200"xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m002941200"xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/author | "Huang W."xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/author | "Huang W."xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/author | "Craik C.S."xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/author | "Craik C.S."xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/author | "Takeuchi T."xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/author | "Takeuchi T."xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/author | "Harris J.L."xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/author | "Harris J.L."xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/author | "Coughlin S.R."xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/author | "Coughlin S.R."xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/author | "Yan K.W."xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/author | "Yan K.W."xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/pages | "26333-26342"xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/pages | "26333-26342"xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/title | "Cellular localization of membrane-type serine protease 1 and identification of protease-activated receptor-2 and single-chain urokinase-type plasminogen activator as substrates."xsd:string |
| http://purl.uniprot.org/citations/10831593 | http://purl.uniprot.org/core/title | "Cellular localization of membrane-type serine protease 1 and identification of protease-activated receptor-2 and single-chain urokinase-type plasminogen activator as substrates."xsd:string |