| http://purl.uniprot.org/citations/10842184 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10842184 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10842184 | http://www.w3.org/2000/01/rdf-schema#comment | "Signal regulatory proteins (SIRPs) are receptor-like transmembrane proteins, the majority of which contain a cytoplasmic proline-rich region and four cytoplasmic tyrosines that, when phosphorylated, bind SH2 domain-containing protein tyrosine phosphatases (SHP). We demonstrated previously that growth hormone (GH) induces tyrosyl phosphorylation of SIRPalpha and association of SIRPalpha with SHP-2. The GH-activated tyrosine kinase JAK2 associates with and tyrosyl-phosphorylates SIRPalpha1. Here we show that JAK2-SIRPalpha1 association does not require phosphotyrosines in SIRPalpha1 or JAK2 or the proline-rich region of SIRPalpha1. However, when the C-terminal 30 amino acids of SIRPalpha1 containing the proline-rich region and tyrosine 495 are deleted, tyrosyl phosphorylation of SIRPalpha1 by JAK2 and association of SHP-2 with SIRPalpha1 are reduced. GH-dependent tyrosyl phosphorylation of JAK2 is reduced when wild-type SIRPalpha1 compared with SIRPalpha1 lacking the four cytoplasmic tyrosines (SIRP 4YF) is expressed in cells, suggesting that SIRPalpha1 negatively regulates GHR/JAK2 signaling. Consistent with reduced JAK2 activity, overexpression of wild-type SIRPalpha1 but not SIRP 4YF reduces GH-induced phosphorylation of ERKs 1 and 2, STAT3, and STAT5B. These results suggest that SIRPalpha1 is a negative regulator of GH signaling and that the ability of SIRPalpha1 mutants to negatively regulate GHR-JAK2 signaling correlates with their ability to bind SHP-2."xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m004238200"xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m004238200"xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/author | "Wang H."xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/author | "Wang H."xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/author | "Ullrich A."xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/author | "Ullrich A."xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/author | "Carter-Su C."xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/author | "Carter-Su C."xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/author | "Argetsinger L.S."xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/author | "Argetsinger L.S."xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/author | "Stofega M.R."xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/author | "Stofega M.R."xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/pages | "28222-28229"xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/pages | "28222-28229"xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/title | "Negative regulation of growth hormone receptor/JAK2 signaling by signal regulatory protein alpha."xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/title | "Negative regulation of growth hormone receptor/JAK2 signaling by signal regulatory protein alpha."xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/volume | "275"xsd:string |
| http://purl.uniprot.org/citations/10842184 | http://purl.uniprot.org/core/volume | "275"xsd:string |