| http://purl.uniprot.org/citations/10850995 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10850995 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10850995 | http://www.w3.org/2000/01/rdf-schema#comment | "We have cloned and sequenced the dit gene cluster encoding enzymes of the catabolic pathway for abietane diterpenoid degradation by Pseudomonas abietaniphila BKME-9. The dit gene cluster is located on a 16.7-kb DNA fragment containing 13 complete open reading frames (ORFs) and 1 partial ORF. The genes ditA1A2A3 encode the alpha and beta subunits and the ferredoxin of the dioxygenase which hydroxylates 7-oxodehydroabietic acid to 7-oxo-11,12-dihydroxy-8, 13-abietadien acid. The dioxygenase mutant strain BKME-941 (ditA1::Tn5) did not grow on nonaromatic abietanes, and transformed palustric and abietic acids to 7-oxodehydroabietic acid in cell suspension assays. Thus, nonaromatic abietanes are aromatized prior to further degradation. Catechol 2,3-dioxygenase activity of xylE transcriptional fusion strains showed induction of ditA1 and ditA3 by abietic, dehydroabietic, and 7-oxodehydroabietic acids, which support the growth of strain BKME-9, as well as by isopimaric and 12, 14-dichlorodehydroabietic acids, which are diterpenoids that do not support the growth of strain BKME-9. In addition to the aromatic-ring-hydroxylating dioxygenase genes, the dit cluster includes ditC, encoding an extradiol ring cleavage dioxygenase, and ditR, encoding an IclR-type transcriptional regulator. Although ditR is not strictly required for the growth of strain BKME-9 on abietanes, a ditR::Km(r) mutation in a ditA3::xylE reporter strain demonstrated that it encodes an inducer-dependent transcriptional activator of ditA3. An ORF with sequence similarity to genes encoding permeases (ditE) is linked with genes involved in abietane degradation."xsd:string |
| http://purl.uniprot.org/citations/10850995 | http://purl.org/dc/terms/identifier | "doi:10.1128/JB.182.13.3784-3793.2000"xsd:string |
| http://purl.uniprot.org/citations/10850995 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.182.13.3784-3793.2000"xsd:string |
| http://purl.uniprot.org/citations/10850995 | http://purl.uniprot.org/core/author | "Mohn W.W."xsd:string |
| http://purl.uniprot.org/citations/10850995 | http://purl.uniprot.org/core/author | "Mohn W.W."xsd:string |
| http://purl.uniprot.org/citations/10850995 | http://purl.uniprot.org/core/author | "Martin V.J."xsd:string |
| http://purl.uniprot.org/citations/10850995 | http://purl.uniprot.org/core/author | "Martin V.J."xsd:string |
| http://purl.uniprot.org/citations/10850995 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10850995 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10850995 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/10850995 | http://purl.uniprot.org/core/name | "J Bacteriol"xsd:string |
| http://purl.uniprot.org/citations/10850995 | http://purl.uniprot.org/core/pages | "3784-3793"xsd:string |
| http://purl.uniprot.org/citations/10850995 | http://purl.uniprot.org/core/pages | "3784-3793"xsd:string |
| http://purl.uniprot.org/citations/10850995 | http://purl.uniprot.org/core/title | "Genetic investigation of the catabolic pathway for degradation of abietane diterpenoids by Pseudomonas abietaniphila BKME-9."xsd:string |
| http://purl.uniprot.org/citations/10850995 | http://purl.uniprot.org/core/title | "Genetic investigation of the catabolic pathway for degradation of abietane diterpenoids by Pseudomonas abietaniphila BKME-9."xsd:string |
| http://purl.uniprot.org/citations/10850995 | http://purl.uniprot.org/core/volume | "182"xsd:string |
| http://purl.uniprot.org/citations/10850995 | http://purl.uniprot.org/core/volume | "182"xsd:string |
| http://purl.uniprot.org/citations/10850995 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10850995 |
| http://purl.uniprot.org/citations/10850995 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10850995 |
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| http://purl.uniprot.org/citations/10850995 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10850995 |
| http://purl.uniprot.org/uniprot/Q7BRJ7 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10850995 |
| http://purl.uniprot.org/uniprot/Q9X4X6 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10850995 |