Results
Your Query
▶
▶
| Subject | Predicate | Object |
|---|---|---|
| http://purl.uniprot.org/citations/10874046 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10874046 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10874046 | http://www.w3.org/2000/01/rdf-schema#comment | "We have completed identification of all the ribosomal proteins (RPs) in spinach plastid (chloroplast) ribosomal 50 S subunit via a proteomic approach using two-dimensional electrophoresis, electroblotting/protein sequencing, high performance liquid chromatography purification, polymerase chain reaction-based screening of cDNA library/nucleotide sequencing, and mass spectrometry (reversed-phase HPLC coupled to electrospray ionization mass spectrometry and electrospray ionization mass spectrometry). Spinach plastid 50 S subunit comprises 33 proteins, of which 31 are orthologues of Escherichia coli RPs and two are plastid-specific RPs (PSRP-5 and PSRP-6) having no homologues in other types of ribosomes. Orthologues of E. coli L25 and L30 are absent in spinach plastid ribosome. 25 of the plastid 50 S RPs are encoded in the nuclear genome and synthesized on cytosolic ribosomes, whereas eight of the plastid RPs are encoded in the plastid organelle genome and synthesized on plastid ribosomes. Sites for transit peptide cleavages in the cytosolic RP precursors and formyl Met processing in the plastid-synthesized RPs were established. Post-translational modifications were observed in several mature plastid RPs, including multiple forms of L10, L18, L31, and PSRP-5 and N-terminal/internal modifications in L2, L11 and L16. Comparison of the RPs in gradient-purified 70 S ribosome with those in the 30 and 50 S subunits revealed an additional protein, in approximately stoichiometric amount, specific to the 70 S ribosome. It was identified to be plastid ribosome recycling factor. Combining with our recent study of the proteins in plastid 30 S subunit (Yamaguchi, K., von Knoblauch, K., and Subramanian, A. R. (2000) J. Biol. Chem. 275, 28455-28465), we show that spinach plastid ribosome comprises 59 proteins (33 in 50 S subunit and 25 in 30 S subunit and ribosome recycling factor in 70 S), of which 53 are E. coli orthologues and 6 are plastid-specific proteins (PSRP-1 to PSRP-6). We propose the hypothesis that PSRPs were evolved to perform functions unique to plastid translation and its regulation, including protein targeting/translocation to thylakoid membrane via plastid 50 S subunit."xsd:string |
| http://purl.uniprot.org/citations/10874046 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m005012200"xsd:string |
| http://purl.uniprot.org/citations/10874046 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m005012200"xsd:string |
| http://purl.uniprot.org/citations/10874046 | http://purl.uniprot.org/core/author | "Yamaguchi K."xsd:string |
| http://purl.uniprot.org/citations/10874046 | http://purl.uniprot.org/core/author | "Yamaguchi K."xsd:string |
| http://purl.uniprot.org/citations/10874046 | http://purl.uniprot.org/core/author | "Subramanian A.R."xsd:string |
| http://purl.uniprot.org/citations/10874046 | http://purl.uniprot.org/core/author | "Subramanian A.R."xsd:string |
| http://purl.uniprot.org/citations/10874046 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10874046 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10874046 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10874046 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10874046 | http://purl.uniprot.org/core/pages | "28466-28482"xsd:string |
| http://purl.uniprot.org/citations/10874046 | http://purl.uniprot.org/core/pages | "28466-28482"xsd:string |
| http://purl.uniprot.org/citations/10874046 | http://purl.uniprot.org/core/title | "The plastid ribosomal proteins. Identification of all the proteins in the 50S subunit of an organelle ribosome (chloroplast)."xsd:string |
| http://purl.uniprot.org/citations/10874046 | http://purl.uniprot.org/core/title | "The plastid ribosomal proteins. Identification of all the proteins in the 50S subunit of an organelle ribosome (chloroplast)."xsd:string |
| http://purl.uniprot.org/citations/10874046 | http://purl.uniprot.org/core/volume | "275"xsd:string |
| http://purl.uniprot.org/citations/10874046 | http://purl.uniprot.org/core/volume | "275"xsd:string |
| http://purl.uniprot.org/citations/10874046 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10874046 |
| http://purl.uniprot.org/citations/10874046 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10874046 |
| http://purl.uniprot.org/citations/10874046 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10874046 |
| http://purl.uniprot.org/citations/10874046 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10874046 |
| http://purl.uniprot.org/uniprot/P27683 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10874046 |
| http://purl.uniprot.org/uniprot/P28805 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10874046 |