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http://purl.uniprot.org/citations/10882114http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10882114http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10882114http://www.w3.org/2000/01/rdf-schema#comment"In the current model for spliceosome assembly, U1 snRNP binds to the 5' splice site in the E complex followed by ATP-dependent binding of U2 snRNP to the branchpoint sequence (BPS) in the A complex. Here we report the characterization of highly purified, functional E complex. We provide evidence that this complex contains functional U2 snRNP and that this snRNP is required for E complex assembly. The BPS is not required for U2 snRNP binding in the E complex. These data suggest a model for spliceosome assembly in which U1 and U2 snRNPs first associate with the spliceosome in the E complex and then an ATP-dependent step results in highly stable U2 snRNP binding to the BPS in the A complex."xsd:string
http://purl.uniprot.org/citations/10882114http://purl.org/dc/terms/identifier"doi:10.1016/s1097-2765(00)80318-4"xsd:string
http://purl.uniprot.org/citations/10882114http://purl.org/dc/terms/identifier"doi:10.1016/s1097-2765(00)80318-4"xsd:string
http://purl.uniprot.org/citations/10882114http://purl.uniprot.org/core/author"Zhou Z."xsd:string
http://purl.uniprot.org/citations/10882114http://purl.uniprot.org/core/author"Zhou Z."xsd:string
http://purl.uniprot.org/citations/10882114http://purl.uniprot.org/core/author"Das R."xsd:string
http://purl.uniprot.org/citations/10882114http://purl.uniprot.org/core/author"Das R."xsd:string
http://purl.uniprot.org/citations/10882114http://purl.uniprot.org/core/author"Reed R."xsd:string
http://purl.uniprot.org/citations/10882114http://purl.uniprot.org/core/author"Reed R."xsd:string
http://purl.uniprot.org/citations/10882114http://purl.uniprot.org/core/date"2000"xsd:gYear
http://purl.uniprot.org/citations/10882114http://purl.uniprot.org/core/date"2000"xsd:gYear
http://purl.uniprot.org/citations/10882114http://purl.uniprot.org/core/name"Mol. Cell"xsd:string
http://purl.uniprot.org/citations/10882114http://purl.uniprot.org/core/name"Mol. Cell"xsd:string
http://purl.uniprot.org/citations/10882114http://purl.uniprot.org/core/pages"779-787"xsd:string
http://purl.uniprot.org/citations/10882114http://purl.uniprot.org/core/pages"779-787"xsd:string
http://purl.uniprot.org/citations/10882114http://purl.uniprot.org/core/title"Functional association of U2 snRNP with the ATP-independent spliceosomal complex E."xsd:string
http://purl.uniprot.org/citations/10882114http://purl.uniprot.org/core/title"Functional association of U2 snRNP with the ATP-independent spliceosomal complex E."xsd:string
http://purl.uniprot.org/citations/10882114http://purl.uniprot.org/core/volume"5"xsd:string
http://purl.uniprot.org/citations/10882114http://purl.uniprot.org/core/volume"5"xsd:string
http://purl.uniprot.org/citations/10882114http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/10882114
http://purl.uniprot.org/citations/10882114http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/10882114
http://purl.uniprot.org/citations/10882114http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/10882114
http://purl.uniprot.org/citations/10882114http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/10882114