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http://purl.uniprot.org/citations/10889249http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/10889249http://www.w3.org/2000/01/rdf-schema#comment"The genomic clone encoding an alpha-tubulin, OsTubA1, has been isolated from rice (Oryza sativa L.). The gene consists of four exons and three introns. RNA-blot analysis showed that the gene is strongly expressed in actively dividing tissues, including root tips, young leaves, and young flowers. Analysis of chimeric fusions between OsTubA1 and beta-glucuronidase (GUS) revealed that the intron 1 was required for high-level GUS expression in actively dividing tissues, corresponding with normal expression pattern of OsTubA1. Fusion constructs lacking the intron 1 showed more GUS staining in mature tissues rather than young tissues. When the intron 1 was placed at the distal region from 5'-upstream region or at the 3'-untranslated region, no enhancement of GUS expression was observed. Sequential deletions of the OsTubA1 intron 1 brought about a gradual reduction of GUS activity in calli. These results suggest that tissue-preferential expression of the OsTubA1 gene is mediated by the intron 1 and that it may be involved in a mechanism for an efficient RNA splicing that is position dependent."xsd:string
http://purl.uniprot.org/citations/10889249http://purl.org/dc/terms/identifier"doi:10.1104/pp.123.3.1005"xsd:string
http://purl.uniprot.org/citations/10889249http://purl.uniprot.org/core/author"An G."xsd:string
http://purl.uniprot.org/citations/10889249http://purl.uniprot.org/core/author"Lee S."xsd:string
http://purl.uniprot.org/citations/10889249http://purl.uniprot.org/core/author"Jung K.H."xsd:string
http://purl.uniprot.org/citations/10889249http://purl.uniprot.org/core/author"Kim C."xsd:string
http://purl.uniprot.org/citations/10889249http://purl.uniprot.org/core/author"Jeon J.S."xsd:string
http://purl.uniprot.org/citations/10889249http://purl.uniprot.org/core/author"Jun S.H."xsd:string
http://purl.uniprot.org/citations/10889249http://purl.uniprot.org/core/date"2000"xsd:gYear
http://purl.uniprot.org/citations/10889249http://purl.uniprot.org/core/name"Plant Physiol"xsd:string
http://purl.uniprot.org/citations/10889249http://purl.uniprot.org/core/pages"1005-1014"xsd:string
http://purl.uniprot.org/citations/10889249http://purl.uniprot.org/core/title"Tissue-preferential expression of a rice alpha-tubulin gene, OsTubA1, mediated by the first intron."xsd:string
http://purl.uniprot.org/citations/10889249http://purl.uniprot.org/core/volume"123"xsd:string
http://purl.uniprot.org/citations/10889249http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/10889249
http://purl.uniprot.org/citations/10889249http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/10889249
http://purl.uniprot.org/uniprot/#_Q0PVB0-mappedCitation-10889249http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/10889249
http://purl.uniprot.org/uniprot/#_P28752-mappedCitation-10889249http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/10889249
http://purl.uniprot.org/uniprot/P28752http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/10889249
http://purl.uniprot.org/uniprot/Q0PVB0http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/10889249