| http://purl.uniprot.org/citations/10891072 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10891072 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10891072 | http://www.w3.org/2000/01/rdf-schema#comment | "Calcium vector protein (CaVP) from amphioxus is a two-domain, calcium-binding protein (18.3 kDa) of the calmodulin superfamily. Only two of the four EF-hand motifs (sites III and IV) have a significant binding affinity for calcium ions. We determined the solution structure of the domain containing these active sites (C-CaVP: W81-S161), in the Ca(2+)-saturated state, using NMR spectroscopy and restrained molecular dynamics. The tertiary structure is similar to other Ca(2+)-binding domains containing a pair of EF-hand motifs. The apo state has spectroscopic and thermodynamic characteristics of a molten globule, with conserved secondary structure but highly fluctuating tertiary organization. Titration of C-CaVP with Ca(2+) revealed a stepwise ion binding, with a stable equilibrium intermediate in which only site III binds a calcium ion. Despite a highly fluctuating structure of the free site IV, the calcium-bound site III has a persistent structure, with similar secondary elements but different interhelix angle and hydrophobic packing relative to the fully calcium-saturated state."xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi000360z"xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi000360z"xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/author | "Sakamoto H."xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/author | "Sakamoto H."xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/author | "Cox J.A."xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/author | "Cox J.A."xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/author | "Craescu C.T."xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/author | "Craescu C.T."xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/author | "Baladi S."xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/author | "Baladi S."xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/author | "Theret I."xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/author | "Theret I."xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/pages | "7920-7926"xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/pages | "7920-7926"xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/title | "Sequential calcium binding to the regulatory domain of calcium vector protein reveals functional asymmetry and a novel mode of structural rearrangement."xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/title | "Sequential calcium binding to the regulatory domain of calcium vector protein reveals functional asymmetry and a novel mode of structural rearrangement."xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/volume | "39"xsd:string |
| http://purl.uniprot.org/citations/10891072 | http://purl.uniprot.org/core/volume | "39"xsd:string |