| http://purl.uniprot.org/citations/10913176 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10913176 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10913176 | http://www.w3.org/2000/01/rdf-schema#comment | "The tumor suppressor protein p53 is frequently inactivated in tumors. It functions as a transcriptional activator as well as a repressor for a number of viral and cellular promoters transcribed by RNA polymerase II (Pol II) and by RNA Pol III. Moreover, it appears that p53 also suppresses RNA Pol I transcription. In this study, we examined the molecular mechanism of Pol I transcriptional inhibition by p53. We show that wild-type, but not mutant, p53 can repress Pol I transcription from a human rRNA gene promoter in cotransfection assays. Furthermore, we show that recombinant p53 inhibits rRNA transcription in a cell-free transcription system. In agreement with these results, p53-null epithelial cells display an increased Pol I transcriptional activity compared to that of epithelial cells that express p53. However, both cell lines display comparable Pol I factor protein levels. Our biochemical analysis shows that p53 prevents the interaction between SL1 and UBF. Protein-protein interaction assays indicate that p53 binds to SL1, and this interaction is mostly mediated by direct contacts with TATA-binding protein and TAF(I)110. Moreover, template commitment assays show that while the formation of a UBF-SL1 complex can partially relieve the inhibition of transcription, only the assembly of a UBF-SL1-Pol I initiation complex on the rDNA promoter confers substantial protection against p53 inhibition. In summary, our results suggest that p53 represses RNA Pol I transcription by directly interfering with the assembly of a productive transcriptional machinery on the rRNA promoter."xsd:string |
| http://purl.uniprot.org/citations/10913176 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.20.16.5930-5938.2000"xsd:string |
| http://purl.uniprot.org/citations/10913176 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.20.16.5930-5938.2000"xsd:string |
| http://purl.uniprot.org/citations/10913176 | http://purl.uniprot.org/core/author | "Comai L."xsd:string |
| http://purl.uniprot.org/citations/10913176 | http://purl.uniprot.org/core/author | "Comai L."xsd:string |
| http://purl.uniprot.org/citations/10913176 | http://purl.uniprot.org/core/author | "Zhai W."xsd:string |
| http://purl.uniprot.org/citations/10913176 | http://purl.uniprot.org/core/author | "Zhai W."xsd:string |
| http://purl.uniprot.org/citations/10913176 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10913176 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10913176 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/10913176 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/10913176 | http://purl.uniprot.org/core/pages | "5930-5938"xsd:string |
| http://purl.uniprot.org/citations/10913176 | http://purl.uniprot.org/core/pages | "5930-5938"xsd:string |
| http://purl.uniprot.org/citations/10913176 | http://purl.uniprot.org/core/title | "Repression of RNA polymerase I transcription by the tumor suppressor p53."xsd:string |
| http://purl.uniprot.org/citations/10913176 | http://purl.uniprot.org/core/title | "Repression of RNA polymerase I transcription by the tumor suppressor p53."xsd:string |
| http://purl.uniprot.org/citations/10913176 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/10913176 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/10913176 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10913176 |
| http://purl.uniprot.org/citations/10913176 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10913176 |
| http://purl.uniprot.org/citations/10913176 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10913176 |
| http://purl.uniprot.org/citations/10913176 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10913176 |
| http://purl.uniprot.org/uniprot/Q15572 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10913176 |
| http://purl.uniprot.org/uniprot/Q15572#attribution-A1546F3F67E478BDAB2E9340EEBF92E6 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/10913176 |