| http://purl.uniprot.org/citations/10913188 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10913188 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10913188 | http://www.w3.org/2000/01/rdf-schema#comment | "Srp1p (importin alpha) functions as the nuclear localization signal (NLS) receptor in Saccharomyces cerevisiae. The srp1-31 mutant is defective in this nuclear localization function, whereas an srp1-49 mutant exhibits defects that are unrelated to this localization function, as was confirmed by intragenic complementation between the two mutants. RPN11 and STS1 (DBF8) were identified as high-dosage suppressors of the srp1-49 mutation but not of the srp1-31 mutation. We found that Sts1p interacts directly with Srp1p in vitro and also in vivo, as judged by coimmunoprecipitation and two-hybrid analyses. Mutants of Sts1p that cannot interact with Srp1p are incapable of suppressing srp1-49 defects, strongly suggesting that Sts1p functions in a complex with Srp1p. STS1 also interacted with the second suppressor, RPN11, a subunit of the 26S proteasome, in the two-hybrid system. Further, degradation of Ub-Pro-beta-galactosidase, a test substrate for the ubiquitin-proteasome system, was defective in srp1-49 but not in srp1-31. This defect in protein degradation was alleviated by overexpression of either RPN11 or STS1 in srp1-49. These results suggest a role for Srp1p in regulation of protein degradation separate from its well-established role as the NLS receptor."xsd:string |
| http://purl.uniprot.org/citations/10913188 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.20.16.6062-6073.2000"xsd:string |
| http://purl.uniprot.org/citations/10913188 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.20.16.6062-6073.2000"xsd:string |
| http://purl.uniprot.org/citations/10913188 | http://purl.uniprot.org/core/author | "Nomura M."xsd:string |
| http://purl.uniprot.org/citations/10913188 | http://purl.uniprot.org/core/author | "Nomura M."xsd:string |
| http://purl.uniprot.org/citations/10913188 | http://purl.uniprot.org/core/author | "Vu L."xsd:string |
| http://purl.uniprot.org/citations/10913188 | http://purl.uniprot.org/core/author | "Vu L."xsd:string |
| http://purl.uniprot.org/citations/10913188 | http://purl.uniprot.org/core/author | "Tabb M.M."xsd:string |
| http://purl.uniprot.org/citations/10913188 | http://purl.uniprot.org/core/author | "Tabb M.M."xsd:string |
| http://purl.uniprot.org/citations/10913188 | http://purl.uniprot.org/core/author | "Tongaonkar P."xsd:string |
| http://purl.uniprot.org/citations/10913188 | http://purl.uniprot.org/core/author | "Tongaonkar P."xsd:string |
| http://purl.uniprot.org/citations/10913188 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10913188 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10913188 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/10913188 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/10913188 | http://purl.uniprot.org/core/pages | "6062-6073"xsd:string |
| http://purl.uniprot.org/citations/10913188 | http://purl.uniprot.org/core/pages | "6062-6073"xsd:string |
| http://purl.uniprot.org/citations/10913188 | http://purl.uniprot.org/core/title | "Evidence for separable functions of Srp1p, the yeast homolog of importin alpha (Karyopherin alpha): role for Srp1p and Sts1p in protein degradation."xsd:string |
| http://purl.uniprot.org/citations/10913188 | http://purl.uniprot.org/core/title | "Evidence for separable functions of Srp1p, the yeast homolog of importin alpha (Karyopherin alpha): role for Srp1p and Sts1p in protein degradation."xsd:string |
| http://purl.uniprot.org/citations/10913188 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/10913188 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/10913188 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10913188 |
| http://purl.uniprot.org/citations/10913188 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10913188 |