| http://purl.uniprot.org/citations/10913825 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10913825 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10913825 | http://www.w3.org/2000/01/rdf-schema#comment | "We have identified two genomic DNA fragments from the human pathogenic fungi, Candida albicans (CaVIG9) and Candida glabrata (CgVIG9) that encode GDP-mannose pyrophosphorylase, a key enzyme for protein glycosylation. The VIG9 homologues of CaVIG9 and CgVIG9 complement an identified protein glycosylation-defective mutation, vig9, of Saccharomyces cerevisiae. The nucleotide sequences of the ORFs, which are 83 and 90% identical to that of the ScVIG9 protein, respectively, showed a predicted gene product homologous to S. cerevisiae GDP-mannose pyrophosphorylase. We examined the enzyme activity of a glutathione S-transferase fusion of each VIG9 gene to synthesize GDP mannose in the cell extracts of a heterologous Escherichia coli expression system. We also developed a method for detecting the enzyme activity using a non-radioactive substrate that would be applicable to high throughput screening."xsd:string |
| http://purl.uniprot.org/citations/10913825 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0304-4165(00)00075-1"xsd:string |
| http://purl.uniprot.org/citations/10913825 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0304-4165(00)00075-1"xsd:string |
| http://purl.uniprot.org/citations/10913825 | http://purl.uniprot.org/core/author | "Chibana H."xsd:string |
| http://purl.uniprot.org/citations/10913825 | http://purl.uniprot.org/core/author | "Chibana H."xsd:string |
| http://purl.uniprot.org/citations/10913825 | http://purl.uniprot.org/core/author | "Ohta A."xsd:string |
| http://purl.uniprot.org/citations/10913825 | http://purl.uniprot.org/core/author | "Ohta A."xsd:string |
| http://purl.uniprot.org/citations/10913825 | http://purl.uniprot.org/core/author | "Arisawa M."xsd:string |
| http://purl.uniprot.org/citations/10913825 | http://purl.uniprot.org/core/author | "Arisawa M."xsd:string |
| http://purl.uniprot.org/citations/10913825 | http://purl.uniprot.org/core/author | "Sudoh M."xsd:string |
| http://purl.uniprot.org/citations/10913825 | http://purl.uniprot.org/core/author | "Sudoh M."xsd:string |
| http://purl.uniprot.org/citations/10913825 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10913825 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10913825 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/10913825 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/10913825 | http://purl.uniprot.org/core/pages | "265-272"xsd:string |
| http://purl.uniprot.org/citations/10913825 | http://purl.uniprot.org/core/pages | "265-272"xsd:string |
| http://purl.uniprot.org/citations/10913825 | http://purl.uniprot.org/core/title | "The VIG9 gene products from the human pathogenic fungi Candida albicans and Candida glabrata encode GDP-mannose pyrophosphorylase."xsd:string |
| http://purl.uniprot.org/citations/10913825 | http://purl.uniprot.org/core/title | "The VIG9 gene products from the human pathogenic fungi Candida albicans and Candida glabrata encode GDP-mannose pyrophosphorylase."xsd:string |
| http://purl.uniprot.org/citations/10913825 | http://purl.uniprot.org/core/volume | "1475"xsd:string |
| http://purl.uniprot.org/citations/10913825 | http://purl.uniprot.org/core/volume | "1475"xsd:string |
| http://purl.uniprot.org/citations/10913825 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10913825 |
| http://purl.uniprot.org/citations/10913825 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10913825 |