| http://purl.uniprot.org/citations/10922363 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10922363 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10922363 | http://www.w3.org/2000/01/rdf-schema#comment | "Here we report the molecular identification of cytosolic glutathione (GSH)-dependent prostaglandin (PG) E(2) synthase (cPGES), a terminal enzyme of the cyclooxygenase (COX)-1-mediated PGE(2) biosynthetic pathway. GSH-dependent PGES activity in the cytosol of rat brains, but not of other tissues, increased 3-fold after lipopolysaccharide (LPS) challenge. Peptide microsequencing of purified enzyme revealed that it was identical to p23, which is reportedly the weakly bound component of the steroid hormone receptor/hsp90 complex. Recombinant p23 expressed in Escherichia coli and 293 cells exhibited all the features of PGES activity detected in rat brain cytosol. A tyrosine residue near the N terminus (Tyr(9)), which is known to be critical for the activity of cytosolic GSH S-transferases, was essential for PGES activity. The expression of cPGES/p23 was constitutive and was unaltered by proinflammatory stimuli in various cells and tissues, except that it was increased significantly in rat brain after LPS treatment. cPGES/p23 was functionally linked with COX-1 in marked preference to COX-2 to produce PGE(2) from exogenous and endogenous arachidonic acid, the latter being supplied by cytosolic phospholipase A(2) in the immediate response. Thus, functional coupling between COX-1 and cPGES/p23 may contribute to production of the PGE(2) that plays a role in maintenance of tissue homeostasis."xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m003504200"xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m003504200"xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/author | "Murakami M."xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/author | "Murakami M."xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/author | "Nakatani Y."xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/author | "Nakatani Y."xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/author | "Tanioka T."xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/author | "Tanioka T."xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/author | "Kudo I."xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/author | "Kudo I."xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/author | "Semmyo N."xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/author | "Semmyo N."xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/pages | "32775-32782"xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/pages | "32775-32782"xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/title | "Molecular identification of cytosolic prostaglandin E2 synthase that is functionally coupled with cyclooxygenase-1 in immediate prostaglandin E2 biosynthesis."xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/title | "Molecular identification of cytosolic prostaglandin E2 synthase that is functionally coupled with cyclooxygenase-1 in immediate prostaglandin E2 biosynthesis."xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/volume | "275"xsd:string |
| http://purl.uniprot.org/citations/10922363 | http://purl.uniprot.org/core/volume | "275"xsd:string |