| http://purl.uniprot.org/citations/10930737 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10930737 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10930737 | http://www.w3.org/2000/01/rdf-schema#comment | "The gene (pykA) encoding pyridoxal kinase which converts pyridoxal (vitamin B(6)) to pyridoxal phosphate was isolated from Dictyostelium discoideum using insertional mutagenesis. Cells of a pykA gene knockout grew poorly in axenic medium with low yield but growth was restored by the addition of pyridoxal phosphate. Sequencing indicated a gene, with one intron, encoding a predicted protein of 301 amino acids that was 42% identical in amino acid sequence to human pyridoxal kinase. After expression of the wild-type gene in Escherichia coli, the purified PykA protein product was shown to have pyridoxal kinase enzymatic activity with a K(m) of 8.7 microM for pyridoxal. Transformation of the Dictyostelium knockout mutant with the human pyridoxal kinase gene gave almost the same level of complementation as that seen using transformation with the wild-type Dictyostelium gene. Phylogenetic analysis indicated that the Dictyostelium amino acid sequence was closer to human pyridoxal kinase than to pyridoxal kinases of lower eukaryotes."xsd:string |
| http://purl.uniprot.org/citations/10930737 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1574-6968.2000.tb09229.x"xsd:string |
| http://purl.uniprot.org/citations/10930737 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1574-6968.2000.tb09229.x"xsd:string |
| http://purl.uniprot.org/citations/10930737 | http://purl.uniprot.org/core/author | "Guo K."xsd:string |
| http://purl.uniprot.org/citations/10930737 | http://purl.uniprot.org/core/author | "Guo K."xsd:string |
| http://purl.uniprot.org/citations/10930737 | http://purl.uniprot.org/core/author | "Newell P.C."xsd:string |
| http://purl.uniprot.org/citations/10930737 | http://purl.uniprot.org/core/author | "Newell P.C."xsd:string |
| http://purl.uniprot.org/citations/10930737 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10930737 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10930737 | http://purl.uniprot.org/core/name | "FEMS Microbiol. Lett."xsd:string |
| http://purl.uniprot.org/citations/10930737 | http://purl.uniprot.org/core/name | "FEMS Microbiol. Lett."xsd:string |
| http://purl.uniprot.org/citations/10930737 | http://purl.uniprot.org/core/pages | "195-200"xsd:string |
| http://purl.uniprot.org/citations/10930737 | http://purl.uniprot.org/core/pages | "195-200"xsd:string |
| http://purl.uniprot.org/citations/10930737 | http://purl.uniprot.org/core/title | "Pyridoxal kinase knockout of Dictyostelium complemented by the human homologue."xsd:string |
| http://purl.uniprot.org/citations/10930737 | http://purl.uniprot.org/core/title | "Pyridoxal kinase knockout of Dictyostelium complemented by the human homologue."xsd:string |
| http://purl.uniprot.org/citations/10930737 | http://purl.uniprot.org/core/volume | "189"xsd:string |
| http://purl.uniprot.org/citations/10930737 | http://purl.uniprot.org/core/volume | "189"xsd:string |
| http://purl.uniprot.org/citations/10930737 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10930737 |
| http://purl.uniprot.org/citations/10930737 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10930737 |
| http://purl.uniprot.org/citations/10930737 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10930737 |
| http://purl.uniprot.org/citations/10930737 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10930737 |
| http://purl.uniprot.org/uniprot/Q55EK9 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10930737 |
| http://purl.uniprot.org/embl-cds/AAG01573.1 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/10930737 |