| http://purl.uniprot.org/citations/10944123 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10944123 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10944123 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/10944123 | http://www.w3.org/2000/01/rdf-schema#comment | "Glycosylphosphatidylinositols (GPIs) are attached to the C-termini of many proteins, thereby acting as membrane anchors. Biosynthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), which transfers N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol. GPI-GnT is a uniquely complex glycosyltransferase, consisting of at least four proteins, PIG-A, PIG-H, PIG-C and GPI1. Here, we report that GPI-GnT requires another component, termed PIG-P, and that DPM2, which regulates dolichol-phosphate-mannose synthase, also regulates GPI-GnT. PIG-P, a 134-amino acid protein having two hydrophobic domains, associates with PIG-A and GPI1. PIG-P is essential for GPI-GnT since a cell lacking PIG-P is GPI-anchor negative. DPM2, but not two other components of dolichol-phosphate-mannose synthase, associates with GPI-GnT through interactions with PIG-A, PIG-C and GPI1. Lec15 cell, a null mutant of DPM2, synthesizes early GPI intermediates, indicating that DPM2 is not essential for GPI-GnT; however, the enzyme activity is enhanced 3-fold in the presence of DPM2. These results reveal new essential and regulatory components of GPI-GnT and imply co-regulation of GPI-GnT and the dolichol-phosphate-mannose synthase that generates a mannosyl donor for GPI."xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/19.16.4402"xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/19.16.4402"xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.uniprot.org/core/author | "Murakami Y."xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.uniprot.org/core/author | "Murakami Y."xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.uniprot.org/core/author | "Maeda Y."xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.uniprot.org/core/author | "Maeda Y."xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.uniprot.org/core/author | "Inoue N."xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.uniprot.org/core/author | "Inoue N."xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.uniprot.org/core/author | "Kinoshita T."xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.uniprot.org/core/author | "Kinoshita T."xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.uniprot.org/core/author | "Watanabe R."xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.uniprot.org/core/author | "Watanabe R."xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.uniprot.org/core/author | "Kangawa K."xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.uniprot.org/core/author | "Kangawa K."xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.uniprot.org/core/author | "Hino J."xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.uniprot.org/core/author | "Hino J."xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.uniprot.org/core/author | "Julius M."xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.uniprot.org/core/author | "Julius M."xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.uniprot.org/core/author | "Marmor M.D."xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.uniprot.org/core/author | "Marmor M.D."xsd:string |
| http://purl.uniprot.org/citations/10944123 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |