| http://purl.uniprot.org/citations/10952992 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10952992 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10952992 | http://www.w3.org/2000/01/rdf-schema#comment | "The cAMP responsive element-binding protein (CREB) is central to second messenger regulated transcription. To elucidate the structural mechanisms of DNA binding and selective dimerization of CREB, we determined to 3.0 A resolution, the structure of the CREB bZIP (residues 283-341) bound to a 21-base pair deoxynucleotide that encompasses the canonical 8-base pair somatostatin cAMP response element (SSCRE). The CREB dimer is stabilized in part by ionic interactions from Arg(314) to Glu(319') and Glu(328) to Lys(333') as well as a hydrogen bond network that links the carboxamide side chains of Gln(322')-Asn(321)-Asn(321')-Gln(322). Critical to family selective dimerization are intersubunit hydrogen bonds between basic region residue Tyr(307) and leucine zipper residue Glu(312), which are conserved in all CREB/CREM/ATF-1 family members. Strikingly, the structure reveals a hexahydrated Mg(2+) ion bound in the cavity between the basic region and SSCRE that makes a water-mediated DNA contact. DNA binding studies demonstrate that Mg(2+) ions enhance CREB bZIP:SSCRE binding by more than 25-fold and suggest a possible physiological role for this ion in somatostatin cAMP response element and potentially other CRE-mediated gene expression."xsd:string |
| http://purl.uniprot.org/citations/10952992 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m007293200"xsd:string |
| http://purl.uniprot.org/citations/10952992 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m007293200"xsd:string |
| http://purl.uniprot.org/citations/10952992 | http://purl.uniprot.org/core/author | "Brennan R.G."xsd:string |
| http://purl.uniprot.org/citations/10952992 | http://purl.uniprot.org/core/author | "Brennan R.G."xsd:string |
| http://purl.uniprot.org/citations/10952992 | http://purl.uniprot.org/core/author | "Schumacher M.A."xsd:string |
| http://purl.uniprot.org/citations/10952992 | http://purl.uniprot.org/core/author | "Schumacher M.A."xsd:string |
| http://purl.uniprot.org/citations/10952992 | http://purl.uniprot.org/core/author | "Goodman R.H."xsd:string |
| http://purl.uniprot.org/citations/10952992 | http://purl.uniprot.org/core/author | "Goodman R.H."xsd:string |
| http://purl.uniprot.org/citations/10952992 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10952992 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10952992 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10952992 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/10952992 | http://purl.uniprot.org/core/pages | "35242-35247"xsd:string |
| http://purl.uniprot.org/citations/10952992 | http://purl.uniprot.org/core/pages | "35242-35247"xsd:string |
| http://purl.uniprot.org/citations/10952992 | http://purl.uniprot.org/core/title | "The structure of a CREB bZIP.somatostatin CRE complex reveals the basis for selective dimerization and divalent cation-enhanced DNA binding."xsd:string |
| http://purl.uniprot.org/citations/10952992 | http://purl.uniprot.org/core/title | "The structure of a CREB bZIP.somatostatin CRE complex reveals the basis for selective dimerization and divalent cation-enhanced DNA binding."xsd:string |
| http://purl.uniprot.org/citations/10952992 | http://purl.uniprot.org/core/volume | "275"xsd:string |
| http://purl.uniprot.org/citations/10952992 | http://purl.uniprot.org/core/volume | "275"xsd:string |
| http://purl.uniprot.org/citations/10952992 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10952992 |
| http://purl.uniprot.org/citations/10952992 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10952992 |
| http://purl.uniprot.org/citations/10952992 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10952992 |
| http://purl.uniprot.org/citations/10952992 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10952992 |