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| http://purl.uniprot.org/citations/10954430 | http://www.w3.org/2000/01/rdf-schema#comment | "We have applied fluorescence ratio imaging to the analysis of an actin-binding protein concentration relative to F-actin in macrophages, in order to explore the role of a novel (alpha)-actinin isoform, actinin-4, relative to that of the classical isoform, actinin-1. Conventional immunofluorescence images showed that both isoforms were enriched in F-actin-rich regions such as cell surface ruffles. However, ratio images further demonstrated that actinin-4 concentrations relative to F-actin were higher in peripheral inward curved ruffles and dorsal circular ruffles, presumed precursor forms of macropinosomes, than in straight linear ruffles, while actinin-1 concentrations were uniform among the different types of ruffles. Macropinosome pulse-labeling and chase experiments indicated that actinin-4 was also closely associated with newly formed macropinosomes and gradually dissociated with their maturation. Consistent with ratio imaging data, macrophages scrape-loaded with anti-actinin-4 showed a more reduced rate of macropinocytosis than those loaded with anti-actinin-1. Altogether, these results indicate that actinin-4 and actinin-1 contribute differently to F-actin dynamics, that actinin-4 is more preferentially involved in early stages of macropinocytosis than actinin-1. A similar redistribution of actinin-4 was also observed during phagocytosis, suggesting that actinin-4 may play the same role in the two mechanistically analogous types of endocytosis, i.e. macropinocytosis and phagocytosis."xsd:string |
| http://purl.uniprot.org/citations/10954430 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.113.18.3329"xsd:string |
| http://purl.uniprot.org/citations/10954430 | http://purl.uniprot.org/core/author | "Araki N."xsd:string |
| http://purl.uniprot.org/citations/10954430 | http://purl.uniprot.org/core/author | "Yamada T."xsd:string |
| http://purl.uniprot.org/citations/10954430 | http://purl.uniprot.org/core/author | "Hirohashi S."xsd:string |
| http://purl.uniprot.org/citations/10954430 | http://purl.uniprot.org/core/author | "Hatae T."xsd:string |
| http://purl.uniprot.org/citations/10954430 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10954430 | http://purl.uniprot.org/core/name | "J Cell Sci"xsd:string |
| http://purl.uniprot.org/citations/10954430 | http://purl.uniprot.org/core/pages | "3329-3340"xsd:string |
| http://purl.uniprot.org/citations/10954430 | http://purl.uniprot.org/core/title | "Actinin-4 is preferentially involved in circular ruffling and macropinocytosis in mouse macrophages: analysis by fluorescence ratio imaging."xsd:string |
| http://purl.uniprot.org/citations/10954430 | http://purl.uniprot.org/core/volume | "113"xsd:string |
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