| http://purl.uniprot.org/citations/10969813 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10969813 | http://www.w3.org/2000/01/rdf-schema#comment | "Azoxymethane (AOM)-induced colonic carcinogenesis involves a number of mutations, including those in the K-ras gene and CTNNB1, that codes for beta-catenin. Prior in vitro studies have also demonstrated that wild type p21(K-ras) can be activated by epigenetic events. We identified 15 K-ras mutations in 14 of 84 AOM-induced colonic tumors by three independent methods. By single strand conformational polymorphism, we also observed mutations in 22 of 68 tumors in exon 3 of CTNNB1. A highly sensitive method was then used to measure p21ras activation levels. All tumors assayed possessing K-ras mutations had significantly higher p21ras activation levels (8.8 +/-1.5%; n = 13) compared with that of control colon (3.7 +/-0.4; n = 6; P < 0.05) or tumors without such mutations (4.2 +/-0.4%; n = 70; P < 0.05). Among tumors with wild-type K-ras, there was a subset of tumors (18 of 70) that had significantly higher p21ras activation levels (8.0 +/-0.9%; n = 18) compared with control colons. In three of four tumors examined with activated wild-type p21ras, we observed increased c-erbB-2 receptor expression and decreased Ras-GAP expression. In contrast, only one of eight tumors examined with wild-type ras and nonactivated p21ras demonstrated these alterations. Mitogen-activated protein kinase (MAPK) activation and cyclooxygenase-2 (COX-2) expression were increased in tumors with mutated or activated wild-type p21ras, compared with their nonactivated counterparts. Although beta-catenin mutations did not alter COX-2 expression or MAPK activity, mutations in either K-ras or beta-catenin significantly increased cyclin D1 expression. In contrast, in tumors with wild-type but activated p21-ras, cyclin D1 expression was not enhanced. Thus, the spectrum of changes in MAPK, COX-2, and cyclin D1 is distinct among tumors with ras or beta-catenin mutations or nonmutational activation of p21ras."xsd:string |
| http://purl.uniprot.org/citations/10969813 | http://purl.uniprot.org/core/author | "Nguyen L."xsd:string |
| http://purl.uniprot.org/citations/10969813 | http://purl.uniprot.org/core/author | "Zhang Y."xsd:string |
| http://purl.uniprot.org/citations/10969813 | http://purl.uniprot.org/core/author | "Bissonnette M."xsd:string |
| http://purl.uniprot.org/citations/10969813 | http://purl.uniprot.org/core/author | "Khare S."xsd:string |
| http://purl.uniprot.org/citations/10969813 | http://purl.uniprot.org/core/author | "Hart J."xsd:string |
| http://purl.uniprot.org/citations/10969813 | http://purl.uniprot.org/core/author | "Varki N."xsd:string |
| http://purl.uniprot.org/citations/10969813 | http://purl.uniprot.org/core/author | "Boss G.R."xsd:string |
| http://purl.uniprot.org/citations/10969813 | http://purl.uniprot.org/core/author | "von Lintig F.C."xsd:string |
| http://purl.uniprot.org/citations/10969813 | http://purl.uniprot.org/core/author | "Brasitus T.A."xsd:string |
| http://purl.uniprot.org/citations/10969813 | http://purl.uniprot.org/core/author | "Skarosi S."xsd:string |
| http://purl.uniprot.org/citations/10969813 | http://purl.uniprot.org/core/author | "Wali R.K."xsd:string |
| http://purl.uniprot.org/citations/10969813 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10969813 | http://purl.uniprot.org/core/name | "Cancer Res"xsd:string |
| http://purl.uniprot.org/citations/10969813 | http://purl.uniprot.org/core/pages | "4602-4609"xsd:string |
| http://purl.uniprot.org/citations/10969813 | http://purl.uniprot.org/core/title | "Mutational and nonmutational activation of p21ras in rat colonic azoxymethane-induced tumors: effects on mitogen-activated protein kinase, cyclooxygenase-2, and cyclin D1."xsd:string |
| http://purl.uniprot.org/citations/10969813 | http://purl.uniprot.org/core/volume | "60"xsd:string |
| http://purl.uniprot.org/citations/10969813 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10969813 |
| http://purl.uniprot.org/citations/10969813 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10969813 |
| http://purl.uniprot.org/uniprot/#_A0JN17-mappedCitation-10969813 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10969813 |
| http://purl.uniprot.org/uniprot/#_P08644-mappedCitation-10969813 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10969813 |
| http://purl.uniprot.org/uniprot/P08644 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/10969813 |
| http://purl.uniprot.org/uniprot/A0JN17 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/10969813 |