| http://purl.uniprot.org/citations/10982866 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10982866 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10982866 | http://www.w3.org/2000/01/rdf-schema#comment | "Replication Protein A (RPA) is required for DNA recombination, repair and replication in all eukaryotes. RPA participation in these pathways is mediated by single-stranded DNA binding and protein interactions. We herein identify a novel protein, Replication Protein Binding Trans-Activator (RBT1), in a yeast two-hybrid assay employing the second subunit of human RPA (RPA32) as bait. RBT1-RPA32 binding was confirmed by glutathione S:-transferase pull-down and co-immunoprecipitation. Fluorescence microscopy indicates that green fluorescence protein-tagged RBT1 is localized to the nucleus in vivo. RBT1 mRNA expression, determined by semi-quantitative RT-PCR, is significantly higher in cancer cell lines MCF-7, ZR-75, SaOS-2 and H661, compared to the cell lines normal non-immortalized human mammary epithelial cells and normal non-immortalized human bronchial epithelial cells. Further, yeast and mammalian one-hybrid analysis shows that RBT1 is a strong transcriptional co-activator. Interestingly, mammalian transactivation data is indicative of significant variance between cell lines; the GAL4-RBT1 fusion protein has significantly higher transcriptional activity in human cancer cells compared to human normal primary non-immortalized epithelial cells. We propose that RBT1 is a novel transcriptional co-activator that interacts with RPA, and has significantly higher activity in transformed cells."xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/28.18.3478"xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/28.18.3478"xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/author | "Bergeron J."xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/author | "Bergeron J."xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/author | "Cho J.M."xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/author | "Cho J.M."xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/author | "Wold M.S."xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/author | "Wold M.S."xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/author | "Alaoui-Jamali M.A."xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/author | "Alaoui-Jamali M.A."xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/author | "Benlimame N."xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/author | "Benlimame N."xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/author | "Song D.J."xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/author | "Song D.J."xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/pages | "3478-3485"xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/pages | "3478-3485"xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/title | "RBT1, a novel transcriptional co-activator, binds the second subunit of replication protein A."xsd:string |
| http://purl.uniprot.org/citations/10982866 | http://purl.uniprot.org/core/title | "RBT1, a novel transcriptional co-activator, binds the second subunit of replication protein A."xsd:string |