| http://purl.uniprot.org/citations/10998421 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10998421 | http://www.w3.org/2000/01/rdf-schema#comment | "A major function of tropomyosin (TPM) in nonmuscle cells may be stabilization of F-actin by binding longitudinally along the actin filament axis. However, no clear evidence exists in vitro that TPM can significantly affect the critical concentration of actin. We previously made a polymerization-defective mutant actin, GG (V266G, L267G). This actin will not polymerize alone at 25 degrees C but will in the presence of phalloidin or beryllium fluoride. With beryllium fluoride, but not phalloidin, this polymerization rescue is cold-sensitive. We show here that GG-actin polymerizability was restored by cardiac tropomyosin and yeast TPM1 and TPM2 at 25 degrees C with rescue efficiency inversely proportional to TPM length (TPM2 > TPM1 > cardiac tropomyosin), indicating the importance of the ends in polymerization rescue. In the presence of TPM, the apparent critical concentration of actin is 5.5 microm, 10-15-fold higher than that of wild type actin but well below that of the GG-actin alone (>20 microm). Non N-acetylated TPMs did not rescue GG-actin polymerization. The TPMs did not prevent cold-induced depolymerization of GG F-actin. TPM-dependent GG-actin polymerization did not occur at temperatures below 20 degrees C. Polymerization rescue may depend initially on the capture of unstable GG-F-actin oligomers by the TPM, resulting in the strengthening of actin monomer-monomer contacts along the filament axis."xsd:string |
| http://purl.uniprot.org/citations/10998421 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m007201200"xsd:string |
| http://purl.uniprot.org/citations/10998421 | http://purl.uniprot.org/core/author | "Rubenstein P.A."xsd:string |
| http://purl.uniprot.org/citations/10998421 | http://purl.uniprot.org/core/author | "Wen K.K."xsd:string |
| http://purl.uniprot.org/citations/10998421 | http://purl.uniprot.org/core/author | "Kuang B."xsd:string |
| http://purl.uniprot.org/citations/10998421 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/10998421 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/10998421 | http://purl.uniprot.org/core/pages | "40594-40600"xsd:string |
| http://purl.uniprot.org/citations/10998421 | http://purl.uniprot.org/core/title | "Tropomyosin-dependent filament formation by a polymerization-defective mutant yeast actin (V266G,L267G)."xsd:string |
| http://purl.uniprot.org/citations/10998421 | http://purl.uniprot.org/core/volume | "275"xsd:string |
| http://purl.uniprot.org/citations/10998421 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10998421 |
| http://purl.uniprot.org/citations/10998421 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10998421 |
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| http://purl.uniprot.org/uniprot/#_P40414-mappedCitation-10998421 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/10998421 |
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| http://purl.uniprot.org/uniprot/P40414 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/10998421 |
| http://purl.uniprot.org/uniprot/P17536 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/10998421 |
| http://purl.uniprot.org/uniprot/P60010 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/10998421 |