| http://purl.uniprot.org/citations/11017876 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11017876 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11017876 | http://www.w3.org/2000/01/rdf-schema#comment | "Programmed cell death is essential for organ development and regeneration. To identify molecules relevant for this process, full length cDNA cloning of a short, developmentally regulated murine cDNA fragment, MERM-3, was performed and showed a 1.7 kb mRNA encoding a 45 kDa protein with an ATP/GTP binding motive (P-loop). Sequence analysis revealed an 82% amino acid identity to the human death associated protein 3 (hDAP-3), a positive mediator of apoptosis. The full length sequence being the murine orthologue of hDAP-3 is therefore referred to as mDAP-3. In situ hybridization and northern blot analysis showed an abundant mRNA expression with a pronounced expression in highly proliferative epithelial compartments. For mDAP-3, cytochrome c release and induction of cell death could be demonstrated by overexpression of a mDAP-3/EGFP fusion protein. DAP-3 mediated apoptosis was shown to depend on a functional P-loop. Intracellular localization studies using the mDAP-3/EGFP fusion protein, cell fractionation and protease protection experiments localized mDAP-3 to the mitochondrial matrix. DAP-3, in contrast to cytochrome c, retained its mitochondrial localization during apoptosis induction. A mutant of a putative yeast orthologue of mDAP-3, YGL129c, here referred to as yDAP-3, has been shown to exhibit disrupted mitochondrial function. yDAP-3 deficient mutants could be shown to progressively loose mitochondrial DNA. Loss of mitochondrial DNA in yDAP-3 was partially prevented by transfection of the yDAP-3 deficient mutant with mDAP-3, indicating functional complementation by murine DAP-3 in the yeast system. These data identify mDAP-3 as one of the first proapoptotic factors in the mitochondrial matrix and provide evidence for a critical, evolutionary conserved role of members of the DAP-3 protein family for mitochondrial biogenesis."xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.113.20.3603"xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.113.20.3603"xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/author | "Luckow B."xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/author | "Luckow B."xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/author | "Herrmann J.M."xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/author | "Herrmann J.M."xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/author | "Berger T."xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/author | "Berger T."xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/author | "Brigl M."xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/author | "Brigl M."xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/author | "Kretzler M."xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/author | "Kretzler M."xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/author | "Schloendorff D."xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/author | "Schloendorff D."xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/author | "Vielhauer V."xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/author | "Vielhauer V."xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/name | "J. Cell Sci."xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/name | "J. Cell Sci."xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/pages | "3603-3612"xsd:string |
| http://purl.uniprot.org/citations/11017876 | http://purl.uniprot.org/core/pages | "3603-3612"xsd:string |