| http://purl.uniprot.org/citations/11019817 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11019817 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11019817 | http://www.w3.org/2000/01/rdf-schema#comment | "Group X secretory phospholipase A2 (sPLA2-X) has recently been shown to possess a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Here, we report the purification of mouse pro- and mature forms of sPLA2-X, as well as its expression and biological functions. Purified pro-sPLA2-X was found to possess a propeptide of 11 amino acid residues attached at the NH2-terminals of the mature protein, and showed as little as 8% of the PLA2 activity of the mature form. Limited proteolysis of pro-sPLA2-X with trypsin resulted in the appearance of the mature form with a concomitant increase in PLA2 activity, suggesting a requirement of proteolytic removal of the propeptide for the optimal activity. The expression of sPLA2-X mRNA was detected in various tissues including the lung, thymus, and spleen, and immunohistochemical analysis revealed its expression in splenic macrophages. In the spleen cells, mature sPLA2-X elicited a prompt release of arachidonic acid with significant production of prostaglandin E2 more efficiently than group IB and IIA sPLA2s. In addition, sPLA2-X was identified as a high-affinity ligand for both native and recombinant form of mouse PLA2 receptor (PLA2R). However, there was no significant difference in the sPLA2-X-induced arachidonic acid release responses in the spleen cells between wild-type and PLA2R-deficient mice. These findings strongly suggest that sPLA2-X possesses two distinct biological functions in mice: it elicits a marked release of arachidonic acid from membrane phospholipids leading to the production of lipid mediators based on its enzymatic potency, and it acts as a natural ligand for the PLA2R that has been shown to play a critical role in the production of inflammatory cytokines during endotoxic shock."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.org/dc/terms/identifier | "doi:10.1006/abbi.2000.1977"xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.org/dc/terms/identifier | "doi:10.1006/abbi.2000.1977"xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Fujii N."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Fujii N."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Ikeda M."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Ikeda M."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Nakano K."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Nakano K."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Ono T."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Ono T."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Suzuki N."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Suzuki N."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Yokota Y."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Yokota Y."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Morioka Y."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Morioka Y."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Ishizaki J."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Ishizaki J."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Arita H."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Arita H."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Saiga A."xsd:string |
| http://purl.uniprot.org/citations/11019817 | http://purl.uniprot.org/core/author | "Saiga A."xsd:string |