| http://purl.uniprot.org/citations/11040421 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11040421 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11040421 | http://www.w3.org/2000/01/rdf-schema#comment | "The poly(ADP-ribose) polymerase-like thermozyme purified from Sulfolobus solfataricus was characterised with respect to some physico-chemical properties. The archaeal protein exhibited a scarce electrophoretic mobility at both pH 2.9 and pH 7.5. Determination of the isoelectric point (pI=7.0-7.2) allowed us to understand the reason for the limited migration at pH 7.5, while amino acid composition analysis showed a moderate content of basic residues, which reduced mobility at pH 2.9. With respect to the charge, the archaeal enzyme behaved differently from the eukaryotic thermolabile poly(ADP-ribose) polymerase, described as a basic protein (pI=9.5). Well known inhibitors of the mesophilic polymerase like Zn(2+), nicotinamide and 3-aminobenzamide exerted a smaller effect on the enzyme from S. solfataricus, reducing the activity by at most 50%. Mg(2+) was a positive effector, although in a dose-dependent manner. It influenced the fluorescence spectrum of the archaeal protein, whereas NaCl had no effect."xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1574-6968.2000.tb09351.x"xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1574-6968.2000.tb09351.x"xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/author | "Gambacorta A."xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/author | "Gambacorta A."xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/author | "Farina B."xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/author | "Farina B."xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/author | "Castellano S."xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/author | "Castellano S."xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/author | "De Luca P."xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/author | "De Luca P."xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/author | "Discenza A."xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/author | "Discenza A."xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/author | "Faraone Mennella M.R."xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/author | "Faraone Mennella M.R."xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/author | "Nicolaus B."xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/author | "Nicolaus B."xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/name | "FEMS Microbiol. Lett."xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/name | "FEMS Microbiol. Lett."xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/pages | "9-14"xsd:string |
| http://purl.uniprot.org/citations/11040421 | http://purl.uniprot.org/core/pages | "9-14"xsd:string |