| http://purl.uniprot.org/citations/11041350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11041350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11041350 | http://www.w3.org/2000/01/rdf-schema#comment | "Clostridium aminobutyricum ferments 4-aminobutyrate via succinic semialdehyde, 4-hydroxybutyrate, 4-hydroxybutyryl-CoA and crotonyl-CoA to acetate and butyrate. The genes coding for the enzymes that catalyse the interconversion of these intermediates are arranged in the order abfD (4-hydroxybutyryl-CoA dehydratase), abfT (4-hydroxybutyrate CoA-transferase), and abfH (NAD-dependent 4-hydroxybutyrate dehydrogenase). The genes abfD and abfT were cloned, sequenced and expressed as active enzymes in Escherichia coli. Hence the insertion of the [4Fe-4S]clusters and FAD into the dehydratase required no additional specific protein from C. aminobutyricum. The amino acid sequences of the dehydratase and the CoA-transferase revealed close relationships to proteins deduced from the genomes of Clostridium difficile, Porphyromonas gingivalis and Archaeoglobus fulgidus. In addition the N-terminal part of the dehydratase is related to those of a family of FAD-containing mono-oxygenases from bacteria. The putative assignment in the databank of Cat2 (OrfZ) from Clostridium kluyveri as 4-hydroxybutyrate CoA-transferase, which is thought to be involved in the reductive pathway from succinate to butyrate, was confirmed by sequence comparison with AbfT (57% identity). Furthermore, an acetyl-CoA:4-hydroxybutyrate CoA-transferase activity could be detected in cell-free extracts of C. kluyveri. In contrast to glutaconate CoA-transferase from Acidaminococcus fermentans, mutation studies suggested that the glutamate residue of the motive EXG, which is conserved in many homologues of AbfT, does not form a CoA-ester during catalysis."xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.org/dc/terms/identifier | "doi:10.1007/s002030000195"xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.org/dc/terms/identifier | "doi:10.1007/s002030000195"xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/author | "Buckel W."xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/author | "Buckel W."xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/author | "Linder D."xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/author | "Linder D."xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/author | "Cinkaya I."xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/author | "Cinkaya I."xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/author | "Gerhardt A."xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/author | "Gerhardt A."xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/author | "Huisman G."xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/author | "Huisman G."xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/name | "Arch. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/name | "Arch. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/pages | "189-199"xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/pages | "189-199"xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/title | "Fermentation of 4-aminobutyrate by Clostridium aminobutyricum: cloning of two genes involved in the formation and dehydration of 4-hydroxybutyryl-CoA."xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/title | "Fermentation of 4-aminobutyrate by Clostridium aminobutyricum: cloning of two genes involved in the formation and dehydration of 4-hydroxybutyryl-CoA."xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/volume | "174"xsd:string |
| http://purl.uniprot.org/citations/11041350 | http://purl.uniprot.org/core/volume | "174"xsd:string |