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http://purl.uniprot.org/citations/11042159http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11042159http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11042159http://www.w3.org/2000/01/rdf-schema#comment"In the effort to prepare the mouse full-length cDNA encyclopedia, we previously developed several techniques to prepare and select full-length cDNAs. To increase the number of different cDNAs, we introduce here a strategy to prepare normalized and subtracted cDNA libraries in a single step. The method is based on hybridization of the first-strand, full-length cDNA with several RNA drivers, including starting mRNA as the normalizing driver and run-off transcripts from minilibraries containing highly expressed genes, rearrayed clones, and previously sequenced cDNAs as subtracting drivers. Our method keeps the proportion of full-length cDNAs in the subtracted/normalized library high. Moreover, our method dramatically enhances the discovery of new genes as compared to results obtained by using standard, full-length cDNA libraries. This procedure can be extended to the preparation of full-length cDNA encyclopedias from other organisms."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.org/dc/terms/identifier"doi:10.1101/gr.145100"xsd:string
http://purl.uniprot.org/citations/11042159http://purl.org/dc/terms/identifier"doi:10.1101/gr.145100"xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Carninci P."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Carninci P."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Hayashizaki Y."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Hayashizaki Y."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Itoh M."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Itoh M."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Konno H."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Konno H."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Muramatsu M."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Muramatsu M."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Okazaki Y."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Okazaki Y."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Sugahara Y."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Sugahara Y."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Shibata K."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Shibata K."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Shibata Y."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Shibata Y."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Hayatsu N."xsd:string
http://purl.uniprot.org/citations/11042159http://purl.uniprot.org/core/author"Hayatsu N."xsd:string