http://purl.uniprot.org/citations/11042159 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/11042159 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/11042159 | http://www.w3.org/2000/01/rdf-schema#comment | "In the effort to prepare the mouse full-length cDNA encyclopedia, we previously developed several techniques to prepare and select full-length cDNAs. To increase the number of different cDNAs, we introduce here a strategy to prepare normalized and subtracted cDNA libraries in a single step. The method is based on hybridization of the first-strand, full-length cDNA with several RNA drivers, including starting mRNA as the normalizing driver and run-off transcripts from minilibraries containing highly expressed genes, rearrayed clones, and previously sequenced cDNAs as subtracting drivers. Our method keeps the proportion of full-length cDNAs in the subtracted/normalized library high. Moreover, our method dramatically enhances the discovery of new genes as compared to results obtained by using standard, full-length cDNA libraries. This procedure can be extended to the preparation of full-length cDNA encyclopedias from other organisms."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.org/dc/terms/identifier | "doi:10.1101/gr.145100"xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.org/dc/terms/identifier | "doi:10.1101/gr.145100"xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Carninci P."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Carninci P."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Hayashizaki Y."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Hayashizaki Y."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Itoh M."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Itoh M."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Konno H."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Konno H."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Muramatsu M."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Muramatsu M."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Okazaki Y."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Okazaki Y."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Sugahara Y."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Sugahara Y."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Shibata K."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Shibata K."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Shibata Y."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Shibata Y."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Hayatsu N."xsd:string |
http://purl.uniprot.org/citations/11042159 | http://purl.uniprot.org/core/author | "Hayatsu N."xsd:string |