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http://purl.uniprot.org/citations/11042274http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11042274http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11042274http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Citation
http://purl.uniprot.org/citations/11042274http://www.w3.org/2000/01/rdf-schema#comment"The gamma-subunit of citrate lyase (EC 4.1.3.6) contains the prosthetic group 2'-(5"-phosphoribosyl)-3'-dephospho-CoA and serves as an acyl carrier protein (ACP). We recently showed that in Escherichia coli the proteins CitG and CitX are essential for holo-ACP synthesis and provided evidence that CitG catalyzes the formation of a prosthetic group precursor from ATP and dephospho-CoA, which is subsequently attached via phosphodiester linkage to apo-ACP by CitX. Here we prove that CitG indeed catalyzes the conversion of ATP and dephospho-CoA to adenine and 2'-(5"-triphosphoribosyl)-3'-dephospho-CoA, the predicted precursor of the prosthetic group. Furthermore, this precursor was transferred by CitX to apo-ACP, yielding holo-ACP. Thus, our proposed mechanism for holo-ACP synthesis could be verified."xsd:string
http://purl.uniprot.org/citations/11042274http://purl.org/dc/terms/identifier"doi:10.1016/s0014-5793(00)02105-0"xsd:string
http://purl.uniprot.org/citations/11042274http://purl.org/dc/terms/identifier"doi:10.1016/s0014-5793(00)02105-0"xsd:string
http://purl.uniprot.org/citations/11042274http://purl.org/dc/terms/identifier"doi:10.1016/s0014-5793(00)02105-0"xsd:string
http://purl.uniprot.org/citations/11042274http://purl.uniprot.org/core/author"Bott M."xsd:string
http://purl.uniprot.org/citations/11042274http://purl.uniprot.org/core/author"Bott M."xsd:string
http://purl.uniprot.org/citations/11042274http://purl.uniprot.org/core/author"Dimroth P."xsd:string
http://purl.uniprot.org/citations/11042274http://purl.uniprot.org/core/author"Dimroth P."xsd:string
http://purl.uniprot.org/citations/11042274http://purl.uniprot.org/core/author"Schneider K."xsd:string
http://purl.uniprot.org/citations/11042274http://purl.uniprot.org/core/author"Schneider K."xsd:string
http://purl.uniprot.org/citations/11042274http://purl.uniprot.org/core/date"2000"xsd:gYear
http://purl.uniprot.org/citations/11042274http://purl.uniprot.org/core/date"2000"xsd:gYear
http://purl.uniprot.org/citations/11042274http://purl.uniprot.org/core/name"FEBS Lett."xsd:string
http://purl.uniprot.org/citations/11042274http://purl.uniprot.org/core/name"FEBS Lett."xsd:string
http://purl.uniprot.org/citations/11042274http://purl.uniprot.org/core/pages"165-168"xsd:string
http://purl.uniprot.org/citations/11042274http://purl.uniprot.org/core/pages"165-168"xsd:string
http://purl.uniprot.org/citations/11042274http://purl.uniprot.org/core/title"Identification of triphosphoribosyl-dephospho-CoA as precursor of the citrate lyase prosthetic group."xsd:string
http://purl.uniprot.org/citations/11042274http://purl.uniprot.org/core/title"Identification of triphosphoribosyl-dephospho-CoA as precursor of the citrate lyase prosthetic group."xsd:string
http://purl.uniprot.org/citations/11042274http://purl.uniprot.org/core/volume"483"xsd:string
http://purl.uniprot.org/citations/11042274http://purl.uniprot.org/core/volume"483"xsd:string
http://purl.uniprot.org/citations/11042274http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/11042274
http://purl.uniprot.org/citations/11042274http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/11042274