| http://purl.uniprot.org/citations/11058444 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11058444 | http://www.w3.org/2000/01/rdf-schema#comment | "Granulocyte-macrophage colony-stimulating factor (GM-CSF), a cytokine secreted by lymphohaemopoietic and other cell lineages, is known to influence ovarian cyclicity and embryo development. The aim of this study was to examine the effect of GM-CSF on ovarian follicular cell function using GM-CSF-deficient (GM -/-) mice. Immature GM -/- and GM +/+ mice were stimulated with eCG, and cumulus-oocyte complexes and mural granulosa cells were collected 48 h later. Expression of GM-CSF receptor (GM-CSFR) alpha and beta mRNA subunits by cumulus-oocyte complexes and mural granulosa cells was examined using RT-PCR. Cumulus-oocyte complexes from both genotypes were found to express mRNA for the GM-CSFRalpha-subunit only, while the mural granulosa cells expressed both the alpha and beta receptor subunits. Cumulus-oocyte complexes recovered from GM -/-mice had approximately twice the number of cumulus cells per cumulus-oocyte complex than did those of GM +/+ mice (P < 0.05), even though the growth-promoting activity of denuded GM -/-oocytes was found to be equivalent to that of wild-type oocytes. GM-CSF deficiency was associated with marginally increased DNA synthesis in cumulus cells and significantly (P < 0.05) lower progesterone production by mural granulosa cells recovered from GM -/-compared with those recovered from GM +/+ mice. The addition of rec-mGM-CSF in vitro did not affect DNA synthesis in either cell type or progesterone production by mural granulosa cells, irrespective of GM-CSF status. There was no effect of GM-CSF deficiency on the capacity of FSH and insulin-like growth factor I to stimulate DNA synthesis in cumulus-oocyte complexes (approximately 15- and threefold, respectively) and in mural granulosa cells (approximately two- and threefold, respectively). Taken together, these data show that GM-CSF influences events associated with follicular maturation in mice. The effects of GM-CSF are not exerted directly in granulosa or cumulus cells, but appear to be mediated indirectly, perhaps through the agency of steroidogenesis-regulating secretions of local macrophage populations residing in the theca."xsd:string |
| http://purl.uniprot.org/citations/11058444 | http://purl.org/dc/terms/identifier | "doi:10.1530/jrf.0.1200283"xsd:string |
| http://purl.uniprot.org/citations/11058444 | http://purl.uniprot.org/core/author | "Norman R.J."xsd:string |
| http://purl.uniprot.org/citations/11058444 | http://purl.uniprot.org/core/author | "Robertson S.A."xsd:string |
| http://purl.uniprot.org/citations/11058444 | http://purl.uniprot.org/core/author | "Armstrong D.T."xsd:string |
| http://purl.uniprot.org/citations/11058444 | http://purl.uniprot.org/core/author | "Gilchrist R.B."xsd:string |
| http://purl.uniprot.org/citations/11058444 | http://purl.uniprot.org/core/author | "Ritter L.J."xsd:string |
| http://purl.uniprot.org/citations/11058444 | http://purl.uniprot.org/core/author | "Rowe D.B."xsd:string |
| http://purl.uniprot.org/citations/11058444 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/11058444 | http://purl.uniprot.org/core/name | "J Reprod Fertil"xsd:string |
| http://purl.uniprot.org/citations/11058444 | http://purl.uniprot.org/core/pages | "283-292"xsd:string |
| http://purl.uniprot.org/citations/11058444 | http://purl.uniprot.org/core/title | "Effect of granulocyte-macrophage colony-stimulating factor deficiency on ovarian follicular cell function."xsd:string |
| http://purl.uniprot.org/citations/11058444 | http://purl.uniprot.org/core/volume | "120"xsd:string |
| http://purl.uniprot.org/citations/11058444 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11058444 |
| http://purl.uniprot.org/citations/11058444 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11058444 |
| http://purl.uniprot.org/uniprot/#_Q14AD9-mappedCitation-11058444 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11058444 |
| http://purl.uniprot.org/uniprot/#_P01587-mappedCitation-11058444 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11058444 |
| http://purl.uniprot.org/uniprot/#_Q5SX78-mappedCitation-11058444 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11058444 |
| http://purl.uniprot.org/uniprot/Q14AD9 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11058444 |
| http://purl.uniprot.org/uniprot/P01587 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11058444 |
| http://purl.uniprot.org/uniprot/Q5SX78 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11058444 |