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http://purl.uniprot.org/citations/11076957http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11076957http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11076957http://www.w3.org/2000/01/rdf-schema#comment"We report the deduced amino acid sequences of two alternately spliced isoforms, designated DEFCAP-L and -S, that differ in 44 amino acids and encode a novel member of the mammalian Ced-4 family of apoptosis proteins. Similar to the other mammalian Ced-4 proteins (Apaf-1 and Nod1), DEFCAP contains a caspase recruitment domain (CARD) and a putative nucleotide binding domain, signified by a consensus Walker's A box (P-loop) and B box (Mg(2+)-binding site). Like Nod1, but different from Apaf-1, DEFCAP contains a putative regulatory domain containing multiple leucine-rich repeats (LRR). However, a distinguishing feature of the primary sequence of DEFCAP is that DEFCAP contains at its NH(2) terminus a pyrin-like motif and a proline-rich sequence, possibly involved in protein-protein interactions with Src homology domain 3-containing proteins. By using in vitro coimmunoprecipitation experiments, both long and short isoforms were capable of strongly interacting with caspase-2 and exhibited a weaker interaction with caspase-9. Transient overexpression of full-length DEFCAP-L, but not DEFCAP-S, in breast adenocarcinoma cells MCF7 resulted in significant levels of apoptosis. In vitro death assays with transient overexpression of deletion constructs of both isoforms using beta-galactosidase as a reporter gene in MCF7 cells suggest the following: 1) the nucleotide binding domain may act as a negative regulator of the killing activity of DEFCAP; 2) the LRR/CARD represents a putative constitutively active inducer of apoptosis; 3) the killing activity of LRR/CARD is inhibitable by benzyloxycarbonyl-Val-Ala-Asp (OMe)-fluoromethyl ketone and to a lesser extent by Asp-Glu-Val-Asp (OMe)-fluoromethyl ketone; and 4) the CARD is critical for killing activity of DEFCAP. These results suggest that DEFCAP is a novel member of the mammalian Ced-4 family of proteins capable of inducing apoptosis, and understanding its regulation may elucidate the complex nature of the mammalian apoptosis-promoting machinery."xsd:string
http://purl.uniprot.org/citations/11076957http://purl.org/dc/terms/identifier"doi:10.1074/jbc.m009853200"xsd:string
http://purl.uniprot.org/citations/11076957http://purl.org/dc/terms/identifier"doi:10.1074/jbc.m009853200"xsd:string
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/author"Dilley K.A."xsd:string
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/author"Dilley K.A."xsd:string
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/author"Vincenz C."xsd:string
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/author"Vincenz C."xsd:string
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/author"Guo R.-F."xsd:string
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/author"Guo R.-F."xsd:string
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/author"Hlaing T."xsd:string
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/author"Hlaing T."xsd:string
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/author"Shi M.M."xsd:string
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/author"Shi M.M."xsd:string
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/author"Ward P.A."xsd:string
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/author"Ward P.A."xsd:string
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/author"Loussia J.M."xsd:string
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/author"Loussia J.M."xsd:string
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/author"Morrish T.A."xsd:string
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/author"Morrish T.A."xsd:string
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/date"2001"xsd:gYear
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/date"2001"xsd:gYear
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/11076957http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
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