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http://purl.uniprot.org/citations/11085272 | http://www.w3.org/2000/01/rdf-schema#comment | "In the post-genome sequencing era the functional analysis of newly discovered proteins becomes more and more important. In this report we describe a genetic approach to the post-translational regulation of protein function in Saccharomyces cerevisiae by creating conditional lethal mutants. The yeast ORFs YDL139c, YDL147w, ERG3 and ERG11 were tagged with sequences encoding the hormone-binding domains of mammalian steroid receptors by PCR-mediated, targeted integration into the yeast genome. We found that the function of the chimeric proteins is regulated in a hormone-dependent way. This technique provides another important tool for the functional analysis of the yeast proteome."xsd:string |
http://purl.uniprot.org/citations/11085272 | http://purl.org/dc/terms/identifier | "doi:10.1007/s004380000325"xsd:string |
http://purl.uniprot.org/citations/11085272 | http://purl.uniprot.org/core/author | "Launhardt H."xsd:string |
http://purl.uniprot.org/citations/11085272 | http://purl.uniprot.org/core/author | "Munder T."xsd:string |
http://purl.uniprot.org/citations/11085272 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
http://purl.uniprot.org/citations/11085272 | http://purl.uniprot.org/core/name | "Mol Gen Genet"xsd:string |
http://purl.uniprot.org/citations/11085272 | http://purl.uniprot.org/core/pages | "317-324"xsd:string |
http://purl.uniprot.org/citations/11085272 | http://purl.uniprot.org/core/title | "Post-translational regulation of Saccharomyces cerevisiae proteins tagged with the hormone-binding domains of mammalian nuclear receptors."xsd:string |
http://purl.uniprot.org/citations/11085272 | http://purl.uniprot.org/core/volume | "264"xsd:string |
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